Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:6.2.1.7 (
BAL
)
1,977
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Xanthine oxidase
(xanthine: oxygen oxidoreductase, EC 1.1.3.22), a molybdenum-containing hydroxylase that produces superoxide and uric acid from purine substrates and molecular oxygen, is involved in the oxidative stress underlying several human pathologies including lung diseases. An enzymatic activity similar to
xanthine oxidase
was previously reported in bronchoalveolar lavage fluid of patients with chronic obstructive pulmonary disease (COPD-
BAL
), by fluorometric analysis of DNA unwinding and cytochrome c reduction kinetics. Here we report the detection of
xanthine oxidase
activity products by electron paramagnetic resonance (EPR) in presence of the spin-trap 5,5-dimethyl-1-pyrroline N-oxide (DMPO) and reversed-phase high-performance liquid chromatography (RP-HPLC) in COPD-
BAL
(n = 14, average age of patients 65 years, range 38-81) and
BAL
from healthy nonsmoker controls (n = 6, average age 64 years, range 44-73). Superoxide DMPO adducts were detected in COPD-
BAL
and in an in vitro system containing xanthine and
xanthine oxidase
(XA/XO), but not in
BAL
controls and when superoxide dismutase (SOD, 1000 I.U./ml) was added to COPD-
BAL
. The HPLC analyses after addition of xanthine showed production of uric acid in COPD-
BAL
and in the XA/XO system but not in
BAL
controls. These results support the involvement of
xanthine oxidase
in the mechanisms of superoxide production by
BAL
supernatant, which increases oxidative stress in chronic obstructive pulmonary disease.
...
PMID:Detection of xanthine oxidase activity products by EPR and HPLC in bronchoalveolar lavage fluid from patients with chronic obstructive pulmonary disease. 982 42
