Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:6.2.1.7 (
BAL
)
1,977
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The influence of pH and angiotensinase inhibitors on the in vitro generation of
angiotensin I
during PRA measurements has been investigated. PRA values obtained at pH 5.7 are higher than those obtained at pH 7.4. At pH 5.7, values obtained using diisopropylfluorophosphate (DRP 9 mM) as an angiotensinase inhibitor are higher than values obtained with a mixture of dimercaprol (
BAL
, 1.6 mM) and hydroxyquinoline (8-OHQ, 3 to 4 mM). Since the two methods for inhibiting angiotensinase are completely and equally efficient, it is suggested that these inhibitors might interfere with the renin angiotensinogen reaction. Significant correlations are observed between the PRA values obtained by the different methods which have been studied. Using an incubation pH of 5.7, and
BAL
and 8-OH quinoline as angiotensinase inhibitors, the distribution of PRA values in a population of 124 hospitalized hypertensive patients ingesting a normal sodium diet had been studied, and it has been demonstrated that the sensitivity of this method of measurement can detect small changes in PRA in patients with low renin activity.
...
PMID:Methodologic problems in plasma renin activity measurements. 1 Jul 27
The angiotensinase inhibitor 2,3-dimercaptopropanol (
BAL
) interferes with peptide-antibody binding when certain sensitive antisera are used in
angiotensin I
radioimmunoassay systems. Three of nine antisera tested showed sufficient interference to produce serious errors in data obtained using these antisera together with
BAL
. For PRA determinations in human plasma, at both pH 5.7 and pH 7.3, relationships between different PRA'S are altered, and results of renin stimulation tests are changed in unpredictable ways. Determination of renin concentration in rat plasma does not require use of
BAL
as inhibitor, and it is best avoided. For human plasma renin determinations, use of
BAL
-sensitive antisera should be avoided, since there is no satisfactory way to correct data for the resulting error.
BAL
itself, rather than its oxidation products, is probably the interfering substance. The interference appears to be due to an interaction between
BAL
and the
BAL
-sensitive antiserum. It is not related to the known actions of
BAL
as chelating or reducing agent.
...
PMID:Interference by 2, 3-dimercapto-1-propanol (BAL) in angiotensin I radioimmunoassay. 95 83
Angiotensin converting enzyme (ACE) is present in the endothelial cells of the normal lung where it converts
angiotensin I
to angiotensin II and inactivates bradykinin. It has been suggested that during endothelial injury ACE is sloughed into the blood, and that if the alveolar capillary membrane is injured, also into the alveolar lining fluid. Seven patients with adult respiratory distress syndrome (ARDS), were compared to 11 normal control subjects, nine patients with sarcoidosis, and six with idiopathic pulmonary fibrosis. Total, differential cell counts and ACE determinations were performed on bronchoalveolar lavage fluid in the ARDS group. ACE was detectable in the
BAL
of all but one ARDS patient. It was concluded that
BAL
ACE is elevated in some ARDS patients, especially those with infectious causes of lung injury. Increased ACE may reflect endothelial damage or local increase in ACE production in response to sepsis.
...
PMID:Angiotensin converting enzyme in bronchoalveolar lavage in ARDS. 302 28
