Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:6.2.1.7 (
BAL
)
1,977
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The influence of pH and angiotensinase inhibitors on the in vitro generation of angiotensin I during
PRA
measurements has been investigated.
PRA
values obtained at pH 5.7 are higher than those obtained at pH 7.4. At pH 5.7, values obtained using diisopropylfluorophosphate (DRP 9 mM) as an angiotensinase inhibitor are higher than values obtained with a mixture of dimercaprol (
BAL
, 1.6 mM) and hydroxyquinoline (8-OHQ, 3 to 4 mM). Since the two methods for inhibiting angiotensinase are completely and equally efficient, it is suggested that these inhibitors might interfere with the renin angiotensinogen reaction. Significant correlations are observed between the
PRA
values obtained by the different methods which have been studied. Using an incubation pH of 5.7, and
BAL
and 8-OH quinoline as angiotensinase inhibitors, the distribution of
PRA
values in a population of 124 hospitalized hypertensive patients ingesting a normal sodium diet had been studied, and it has been demonstrated that the sensitivity of this method of measurement can detect small changes in
PRA
in patients with low renin activity.
...
PMID:Methodologic problems in plasma renin activity measurements. 1 Jul 27
The angiotensinase inhibitor 2,3-dimercaptopropanol (
BAL
) interferes with peptide-antibody binding when certain sensitive antisera are used in angiotensin I radioimmunoassay systems. Three of nine antisera tested showed sufficient interference to produce serious errors in data obtained using these antisera together with
BAL
. For
PRA
determinations in human plasma, at both pH 5.7 and pH 7.3, relationships between different
PRA
'S are altered, and results of renin stimulation tests are changed in unpredictable ways. Determination of renin concentration in rat plasma does not require use of
BAL
as inhibitor, and it is best avoided. For human plasma renin determinations, use of
BAL
-sensitive antisera should be avoided, since there is no satisfactory way to correct data for the resulting error.
BAL
itself, rather than its oxidation products, is probably the interfering substance. The interference appears to be due to an interaction between
BAL
and the
BAL
-sensitive antiserum. It is not related to the known actions of
BAL
as chelating or reducing agent.
...
PMID:Interference by 2, 3-dimercapto-1-propanol (BAL) in angiotensin I radioimmunoassay. 95 83
