Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:6.2.1.7 (
BAL
)
1,977
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Two substances, cAMP and 2,3-dimercapto-1-propanol (
BAL
) are known to induce transient activation of
adenylate cyclase
in Dictyostelium discoideum. A frigid mutant (HC85) has a deletion in a gene for G alpha 2, a guanine nucleotide binding protein and cannot activate the cyclase in response to cAMP. We found that
BAL
induced activation in the frigid mutant. This result suggests that the
BAL
-induced activation is independent of G alpha 2 and that
BAL
mimics a role of activated G alpha 2. We also found that cAMP promoted the
BAL
-induced activation. This result suggests that cAMP plays a role in activation through a mechanism in which G alpha 2 is not involved. We lastly showed that continuous cAMP stimulation could not inhibit the
BAL
-induced activation in the frigid mutant. Since the cAMP-induced inhibition observed in the wild type strain (NC4) proceeds with the time course identical to the cAMP-induced adaptation (Oyama, submitted), this result suggests that G alpha 2 is involved in adaptation of
adenylate cyclase
.
...
PMID:Role of a guanine nucleotide binding protein, G alpha 2, in regulation of adenylate cyclase in Dictyostelium discoideum. 190 Jan 54
Binding of cyclic AMP (cAMP) to cell surface receptors induces activation and adaptation of
adenylate cyclase
while 2,3-dimercapto-1-propanol (
BAL
) acts only on the activation pathway. Here we show that an inhibitor of protein kinase (K252a) inhibits the cAMP-induced activation of the cyclase but not (rather enhances) the
BAL
-induced activation. These results suggest that protein kinase is involved in transduction of the activation signal and that phosphorylation might take place between the receptor and the action site of
BAL
. Since adaptation causes cessation of the activation, the enhancement of the
BAL
-induced cAMP accumulation by K252a might imply that K252a also blocks transduction of the adaptation signal.
...
PMID:Involvement of protein kinase(s) in the intracellular signal transduction pathways for activation and adaptation of adenylate cyclase in Dictyostelium discoideum. 215 15
