Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:6.2.1.7 (BAL)
 1,977 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The BAL technique was used to assess the pulmonary injury of coal dust and rock dust in Huai-Nan coal mine in Anhui Province. Dust suspended in saline and dust-free supernatant were instilled intratracheally to Wistar rats and Syrian hamsters. Saline was used in treating controls. The animals were sacrificed 24 h after treatment, their lungs were lavaged, and pulmonary damage was evaluated by cellular and biochemical assays of lavage fluid. Pulmonary injury was expressed by the cell toxicity index (CTI). CTI is the product of the number of times of meaningful parameters for treated groups compared to controls (LDH, acid phosphatase, and polymorphonuclear neutrophils in this case). The CTI values were found to be 5.19, 2.28, and 5.15 for rock dust, coal dust, and Shanghai suspended particulates, respectively. The toxicity of rock dust is higher than that of coal dust, but is similar to that of Shanghai suspended particulates. The cell toxicity of dust suspension solution is higher than that of dust-free supernatant. CTI can be used as an indicator of the relative toxicity of respirable dusts in in vivo studies.
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PMID:Pulmonary injury in laboratory animals induced by Huai-Nan coal mine respirable dust. 327 May 14

Experiments were carried out to determine the long-term effect of instillation of 500 mg of generic bituminous, anthracite, quartz, or titanium dioxide (TiO2) dust on the composition of pulmonary surfactant. Dust was instilled in the caudal lobe of the right lungs of female pigtailed macaque monkeys (Macaca nemestrina). The composition of surfactant isolated from cell-free bronchoalveolar lavage (CF-BAL) samples obtained from right lungs (dust exposed) at various times over the following year was compared with that of surfactant isolated from CF-BAL from left lungs (dust free). Little change was seen in the amount of surfactant-associated lipid phosphorus as a result of exposure to dust. Exposure to quartz, anthracite, or TiO2 dust induced a significant increase in the total amount of protein in the surfactant-enriched fraction. The relative amount of specific proteins was also altered: surfactant-associated protein A decreased, and the amount of the heavy and light chains of immunoglobulin molecules (identified by NH2-terminal amino acid sequence analysis) increased. These changes were visible more than a year after instillation of quartz and at least 3 months after instillation of anthracite dust. Despite variation in the responses of the individual animals, the changes observed might serve as an indicator of the severity of the effect of exposure of the lung to mineral dust and/or to pathogens.
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PMID:Long-term effects of instilled mineral dusts on pulmonary surfactant isolated from monkeys. 761 61