Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:6.2.1.7 (
BAL
)
1,977
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
This investigation was designed to study the effect of thyroidectomy on the release of
thyrotropin-releasing hormone
(
TRH
) from the mediobasal hypothalamus (MBH) of ovariectomized (Ovx) rats in vitro. Immediately after ovariectomy, rats were thyroidectomized (Tx) or sham Tx, and decapitated 10 weeks later. The blood samples were collected, mixed with or without 2,3-dimercaptopropanol (
BAL
). The serum samples with
BAL
were assayed for immunoreactive (IR)
TRH
and those without
BAL
were assayed for thyroid-stimulating hormone (TSH) by radioimmunoassay (RIA). The mediobasal hypothalamus was extracted by 0.1 N HC1, or superfused with Krebs-Ringer phosphate buffer at 37 degrees C in a superfusion chamber, and stimulated by potassium ion (30 mM). The superfusate flowing from the superfusion chamber was collected during successive 10-min intervals at a rate of 0.5 ml/10 min and acidified with 5 N HC1. Concentrations of
TRH
in medium and tissue samples were determined by RIA. Thyroidectomy increased TSH concentration in the serum, but decreased
TRH
concentration in the serum and MBH. Both the spontaneous release and the K(+)-stimulated release of
TRH
from MBHs in vitro were lower in Ovx-Tx than in Ovx rats. These results suggest that the increased secretion of TSH in ovariectomized rats following thyroidectomy can not be attributed to changes of
TRH
secretion from mediobasal hypothalamus.
...
PMID:Effect of thyroidectomy on hypothalamic concentration and in vitro release of thyrotropin-releasing hormone in ovariectomized rats. 211 29
