Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:6.2.1.1 (
ACS
)
78,556
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
In biology, the activity of enzymes is usually regulated by feedback loops, which enables direct communication between enzymes and the state of the cell. In a similar manner, with the intention to have automated activity regulation, the therapeutic effect of a photosensitizer (
BOD1
) is shown to be reduced through a negative feedback loop initiated by the photosensitizer. Photodynamic action produces cytotoxic
1
O
2
and this reactive oxygen species reacts with ascorbate, generating H
2
O
2
. Peroxide-mediated oxidation of the photosensitizer auxiliary group leads to the formation of inactive
BOD2
from the parent photosensitizer.
BOD1
is shown to accumulate in mitochondria, and cell viability is shown to decrease significantly with
BOD1
compared to the loop end product,
BOD2
. Photoinduced enhancement of fluorescence indicates the formation of inactive
BOD2
under cellular conditions, and enhanced fluorescence acts as a reporter for the activity of the photosensitizer. We present the first example of PDT autoinactivation, and such a feedback control mechanism would enable a decrease in post-therapy side effects.
ACS
Omega 2019 Jul 31
PMID:Autoinhibitory Feedback Control over Photodynamic Action. 3146 Mar 46
