Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:6.2.1.1 (
ACS
)
78,556
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
In this work, TiO
2
nanowires synthesized from a wet-corrosion process were presented for peptide sequencing by photocatalytic reaction with UV radiation. For the photocatalytic decomposition of peptides, the peptide sample was dropped on a target plate containing synthesized TiO
2
nanowire zones and UV-irradiated. Subsequently, the target plate was analyzed by laser desorption/ionization time-of-flight (LDI-TOF) mass spectrometry using the synthesized TiO
2
nanowires as a solid matrix. The feasibility of peptide sequencing based on the photocatalytic reaction with the synthesized TiO
2
nanowires was demonstrated using six types of peptides GHP9 (G
1
-H-P-Q-G
2
-K
1
-K
2
-K
3
-K
4
, 1006.59 Da),
BPA-1
(K
1
-S
1
-L-E-N-S
2
-Y-G
1
-G
2
-G
3
-K
2
-K
3
-K
4
, 1394.74 Da), PreS1(F
1
-G-A-N
1
-S-N
2
-N
3
-P
1
-D
1
-W-D
2
-F
2
-N
4
-P
2
-N
5
, 1707.68 Da), HPQ peptide-1 (G-Y-H-P-Q-R-K, 884.45 Da), HPQ peptide-2 (K-R-H-P-Q-Y-G, 884.45 Da), and HPQ peptide-3 (R-Y-H-P-Q-G-K, 884.45 Da). The identification of three different peptides with the same molecular weight was also demonstrated by using the synthesized TiO
2
nanowires for their photocatalytic decomposition as well as for LDI-TOF mass spectrometry as a solid-matrix.
ACS
Appl Mater Interfaces 2018 Oct 10
PMID:TiO
2
Nanowires from Wet-Corrosion Synthesis for Peptide Sequencing Using Laser Desorption/Ionization Time-of-Flight Mass Spectrometry. 3021 81
