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Query: EC:6.2.1.1 (
ACS
)
78,556
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The plant hormone ethylene plays a regulatory role in development in light- and dark-grown seedlings. We previously isolated a group of small-molecule compounds with a quinazolinone backbone, which were named acsinones (for ACC synthase inhibitor quinazolinones), that act as uncompetitive inhibitors of 1-aminocyclopropane-1-carboxylic acid (ACC) synthase (
ACS
). Thus, the triple response phenotype, which consists of shortened hypocotyls and roots, radial swelling of hypocotyls and exaggerated curvature of apical hooks, was suppressed by acsinones in dark-grown (etiolated)
ethylene overproducer
(
eto
) seedlings. Here, we describe our isolation and characterization of an Arabidopsis
revert to eto1 9
(
ret9
) mutant, which showed reduced sensitivity to acsinones in etiolated
eto1
seedlings. Map-based cloning of
RET9
revealed an amino acid substitution in
CHITINASE LIKE1
(
CTL1
), which is required for cell wall biogenesis and stress resistance in Arabidopsis. Etiolated seedlings of
ctl1
ret
9
showed short hypocotyls and roots, which were augmented in combination with
eto1-4
. Consistently,
ctl1
ret
9
seedlings showed enhanced sensitivity to exogenous ACC to suppress primary root elongation as compared with the wild type. After introducing
ctl1
ret
9
to mutants completely insensitive to ethylene, genetic analysis indicated that an intact ethylene response pathway is essential for the alterations in root and apical hook but not hypocotyl in etiolated
ctl1
ret
9
seedlings. Furthermore, a mild yet significantly increased ethylene level in
ctl1
mutants was related to elevated mRNA level and activity of ACC oxidase (ACO). Moreover, genes associated with ethylene biosynthesis (
ACO1
and
ACO2
) and response (
ERF1
and
EDF1
) were upregulated in etiolated
ctl1
ret
9
seedlings. By characterizing a new recessive allele of
CTL1
, we reveal that
CTL1
negatively regulates ACO activity and the ethylene response, which thus contributes to understanding a role for ethylene in root elongation in response to perturbed cell wall integrity.
...
PMID:
CHITINASE LIKE1
Regulates Root Development of Dark-Grown Seedlings by Modulating Ethylene Biosynthesis in
Arabidopsis thaliana
. 3115 71
