Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:6.2.1.1 (
ACS
)
78,556
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Phase pure nondoped and Ce doped La
3
Si
6.5
Al
1.5
N
9.5
O
5.5
(Al containing La N-phase) samples have been obtained by solid-state reaction synthesis for the first time. 1% Ce-doped La
3
Si
6.5
Al
1.5
N
9.5
O
5.5
phosphor displays a broad excitation band ranging from UV to 410 nm, with a maximum at 355 nm. UV light excitation results in a narrow Ce
3+
5d-4f emission band (fwhm = 68 nm) centered at 418 nm. The emission can be tuned from 417 nm at 0.5% Ce to 450 nm at 50% Ce. A high internal quantum efficiency up to 84% is achieved for a 1% Ce doped sample, which has CIE chromaticity coordinates of x = 0.157 and y = 0.069, close to the NTSC blue standard (x = 0.155; y = 0.070). Compared to La
3
Si
8
O
4
N
11
:Ce phosphor, the quantum efficiency and thermal stability have been enhanced for La
3
Si
6.5
Al
1.5
N
9.5
O
5.5
:Ce phosphor without shifting the emission peak wavelength. La
3
Si
6.5
Al
1.5
N
9.5
O
5.5
:Ce shows less thermal quenching than La
3
Si
8
O
4
N
11
:Ce and no shift or change in the shape of emission spectra with increasing the temperature from 4 to 573 K. These results show that La
3
Si
6.5
Al
1.5
N
9.5
O
5.5
:Ce is more efficient than any other (oxy-)nitride phosphor with an emission in the short wavelength blue region (400-450 nm). A white LED was fabricated using the La
3
Si
6.5
Al
1.5
N
9.5
O
5.5
:5%Ce as a blue phosphor. The high color rendering index (Ra = 93.2, R9 = 91.4, and
R12
= 89.5) obtained shows that the phosphor is a very promising conversion phosphor for white LEDs.
ACS
Appl Mater Interfaces 2017 Jul 12
PMID:Ce-Doped La
3
Si
6.5
Al
1.5
N
9.5
O
5.5
, a Rare Highly Efficient Blue-Emitting Phosphor at Short Wavelength toward High Color Rendering White LED Application. 2858 4
Atrazine is an herbicide that is widely used in crop production at about 70 million pounds per year in the United States. Its widespread use has led to contamination of groundwater and other aquatic systems. It has resulted in many serious environmental and human health issues. This study focuses on the identification and characterization of a single-stranded DNA (ssDNA) aptamer that binds to atrazine. In this study, a variation of the systematic evolution of ligands by exponential enrichment (SELEX) process was used to identify an aptamer, which binds to atrazine with high affinity and specificity. This SELEX focused on inducing the aptamer's ability to change conformation upon binding to atrazine, and stringent negative target selections. After 12 rounds of in vitro selection, the ssDNA aptamer candidate
R12
.45 was chosen and truncated to obtain a 46-base sequence. The binding affinity, specificity, and structural characteristics of this truncated candidate was investigated by using isothermal titration calorimetry, circular dichroism (CD) analysis, SYBR Green I (SG) fluorescence displacement assays, and gold nanoparticles (AuNPs) colorimetric assays. The truncated
R12
.45 candidate aptamer bound to atrazine with high affinity (
K
d
= 3.7 nM) and displayed low cross-binding activities on structurally related herbicides. In addition, CD analysis data indicated a target induced structural stabilization. Finally, SG assays and AuNPs assays showed nonconventional binding activities between the truncated
R12
.45 aptamer candidate and atrazine, which warrants future studies.
ACS
Omega 2018 Oct 31
PMID:In Vitro Selection and Characterization of a Single-Stranded DNA Aptamer Against the Herbicide Atrazine. 3041 Oct 44
Atrazine is a common herbicide that is widely used to control weed growth in both agricultural and residential settings. It has been shown to act as an endocrine disruptor that affects aquatic organisms. Rapid and low-cost monitoring methods for atrazine is the first step to mitigate its widespread persistency. Aptamers are small synthetic oligonucleotides that can assume a 3D structure to act as the molecular recognition element for a specific target of interest. Two different atrazine binding aptamers (
R12
.23 Trunc. and
R12
.45 Trunc.) have been identified from the same library design but with fundamentally different in vitro selection methodologies. While the
R12
.23 Trunc. has been utilized in immobilized biosensing platforms, it is unclear if in-solution-based applications would be suitable for both atrazine binding aptamers. This study provides the first insight of comparative in-solution binding profiles of the two atrazine binding aptamers. Based on our results, this information will be useful for future biosensing platform development utilizing the two aptamers.
ACS
Omega 2019 Oct 01
PMID:In-Solution Molecular Recognition Comparison of Aptamers against the Herbicide Atrazine. 3159 87
The common phenomenon that the nonviral vectors have much lower transfection efficiency in vivo than in vitro greatly restricts their further developments and applications. Possible reasons are lacking targeting ability, elimination by the reticuloendothelial system (RES), and insufficient nuclear transport. Here, a novel, flexible, and deformable polymer Fe@PEI-
R12
(tLyp-1-NLS) is reported for shortening the gap between in vitro and in vivo gene transfection efficiency. The amorphous network structure Fe@PEI with deformation ability acquired by coordination cross-linking of Fe
3+
and low-molecular-weight polyethylenimine (LMW-PEI) constructs the core and serves as the gene reservoir, and it can squeeze out through RES filter holes when trapped in the spleen. The bifunctional peptide
R12
provided tumor targeting and enhanced nuclear delivery ability. Additionally, the Fe
3+
from Fe@PEI-
R12
could trigger endogenous hydrogen peroxide (H
2
O
2
) decomposition to produce O
2
, thereby reducing the adverse effects of tumor hypoxia. It is demonstrated that the Fe@PEI-
R12
/pDNA complexes could pass through membrane filters, subsequently achieving long circulation time, and Fe@PEI-
R12
had a tendency to accumulate in tumor tissue and mediate pGL3-control expression. Therefore, the multifunctional nanoplatform has the potential for effective in vivo gene delivery.
ACS
Appl Mater Interfaces 2020 Jan 22
PMID:Fe
3+
-Coordinated Multifunctional Elastic Nanoplatform for Effective in Vivo Gene Transfection. 3189 37
