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Query: EC:6.2.1.1 (
ACS
)
78,556
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
It has recently been described that the Japanese plum "Santa Rosa" bud sport series contains variations in ripening pattern: climacteric, suppressed-climacteric and non-climacteric types. This provides an interesting model to study the role of ethylene and other key mechanisms governing fruit ripening, softening and senescence. The aim of the current study was to investigate such differences at the genomic level, using this series of plum bud sports, with special reference to genes involved in ethylene biosynthesis, signal transduction, and sugar metabolism. Genomic DNA, isolated from leaf samples of six Japanese plum cultivars ("Santa Rosa", "July Santa Rosa", "Late Santa Rosa", "Sweet Miriam", "Roysum", and "Casselman"), was used to construct paired-end standard Illumina libraries. Sequences were aligned to the
Prunus persica
genome, and genomic variations (SNPs, INDELS, and CNV's) were investigated. Results determined 12 potential candidate genes with significant copy number variation (CNV), being associated with ethylene perception and signal transduction components. Additionally, the Maximum Likelihood (ML) phylogenetic tree showed two sorbitol dehydrogenase genes grouping into a distinct clade, indicating that this natural group is well-defined and presents high sequence identity among its members. In contrast, the ethylene group, which includes ACO1, ACS1, ACS4, ACS5, CTR1,
ERF1
, ERF3, and ethylene-receptor genes, was widely distributed and clustered into 10 different groups. Thus,
ACS
, ERF, and sorbitol dehydrogenase proteins potentially share a common ancestor for different plant genomes, while the expansion rate may be related to ancestral expansion rather than species-specific events. Based on the distribution of the clades, we suggest that gene function diversification for the ripening pathway occurred prior to family extension. We herein report all the frameshift mutations in genes involved in sugar transport and ethylene biosynthesis detected as well as the gene CNV implicated in ripening differences.
...
PMID:Genomic Sequencing of Japanese Plum (
Prunus salicina
Lindl.) Mutants Provides a New Model for Rosaceae Fruit Ripening Studies. 2951 96
The plant hormone ethylene plays a regulatory role in development in light- and dark-grown seedlings. We previously isolated a group of small-molecule compounds with a quinazolinone backbone, which were named acsinones (for ACC synthase inhibitor quinazolinones), that act as uncompetitive inhibitors of 1-aminocyclopropane-1-carboxylic acid (ACC) synthase (
ACS
). Thus, the triple response phenotype, which consists of shortened hypocotyls and roots, radial swelling of hypocotyls and exaggerated curvature of apical hooks, was suppressed by acsinones in dark-grown (etiolated)
ethylene overproducer
(
eto
) seedlings. Here, we describe our isolation and characterization of an Arabidopsis
revert to eto1 9
(
ret9
) mutant, which showed reduced sensitivity to acsinones in etiolated
eto1
seedlings. Map-based cloning of
RET9
revealed an amino acid substitution in
CHITINASE LIKE1
(
CTL1
), which is required for cell wall biogenesis and stress resistance in Arabidopsis. Etiolated seedlings of
ctl1
ret
9
showed short hypocotyls and roots, which were augmented in combination with
eto1-4
. Consistently,
ctl1
ret
9
seedlings showed enhanced sensitivity to exogenous ACC to suppress primary root elongation as compared with the wild type. After introducing
ctl1
ret
9
to mutants completely insensitive to ethylene, genetic analysis indicated that an intact ethylene response pathway is essential for the alterations in root and apical hook but not hypocotyl in etiolated
ctl1
ret
9
seedlings. Furthermore, a mild yet significantly increased ethylene level in
ctl1
mutants was related to elevated mRNA level and activity of ACC oxidase (ACO). Moreover, genes associated with ethylene biosynthesis (
ACO1
and
ACO2
) and response (
ERF1
and
EDF1
) were upregulated in etiolated
ctl1
ret
9
seedlings. By characterizing a new recessive allele of
CTL1
, we reveal that CTL1 negatively regulates ACO activity and the ethylene response, which thus contributes to understanding a role for ethylene in root elongation in response to perturbed cell wall integrity.
...
PMID:
CHITINASE LIKE1
Regulates Root Development of Dark-Grown Seedlings by Modulating Ethylene Biosynthesis in
Arabidopsis thaliana
. 3115 71
