Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:6.2.1.1 (
ACS
)
78,556
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
A sensitive virus detection method applicable for an early stage increases the probability of survival. Here, we develop a simple and rapid detection strategy for the detection of the
hepatitis E
virus (HEV) by an electrocatalytic water oxidation reaction (WOR) using a platinum (Pt)-incorporated cobalt (Co)-based zeolite imidazole framework (ZIF-67). The surface cavity of ZIF-67 enables the rich loading of Pt NPs, and subsequent calcination etches the cavity, promoting the electrocatalytic activity of Pt-Co
3
O
4
HCs. The Pt-Co
3
O
4
HCs show excellent behavior for the WOR due to the synergistic interaction of Pt and Co
3
O
4
, evaluated by voltammetry and chronoamperometry. The synthesized Pt-Co
3
O
4
HCs are conjugated with anti-HEV antibody (Ab@Pt-Co
3
O
4
HCs); the electrocatalytic activity of Ab@Pt-Co
3
O
4
HCs is combined with that of antibody-conjugated magnetic nanoparticles (MNPs) for HEV detection by a magneto-and-nanocomposite sandwich immunoassay. The sensor is challenged to detect the HEV in spiked serum samples and HEV G7 genotypes collected from the cell culture supernatant, reaching a low limit of detection down to 61 RNA copies mL
-1
. This work establishes a free-indicator one-step approach with the controlled design of Pt-Co
3
O
4
HCs, which presents an effective WOR technique for virus detection in a neutral pH solution, which can be extended to electrocatalytic studies in the future integrated biosensing systems.
ACS
Appl Mater Interfaces 2020 Nov 11
PMID:Ultrasensitive Detection of the Hepatitis E Virus by Electrocatalytic Water Oxidation Using Pt-Co
3
O
4
Hollow Cages. 3296 16
