Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:6.1.1.10 (
methionyl-tRNA synthetase
)
387
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The single-stranded region of the amino acid acceptor stem corresponding to the 3'-end of E. coli tRNAMetf was replaced by ligation of chemically synthesized ribooligonucleotides, in order to change the length of the single-stranded CCA terminus. The chemically synthesized ribooligomers, CCA,
ACCA
, AACCA and CAACCA, were ligated to nuclease-treated E. coli tRNAMetf, which lacked the
ACCA
sequence at the 3'-end. The methionine acceptor activities of these modified tRNAs were examined using E. coli
methionyl-tRNA synthetase
. Ligation of the chemically synthesized pentamer (AACCA) to the acceptor terminus restored the methionine acceptor activity, whereas ligation of the hexamer (CAACCA) or trimer (CCA) to the acceptor terminus did not Modification of the acceptor terminus had no effect on the formylation of accepted methionine.
...
PMID:Modification of the amino acid acceptor stem of E. coli tRNAMetf by ligation of chemically synthesized ribooligonucleotides. 241 92
