Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:5.99.1.2 (
topoisomerase
)
9,166
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Deoxyribonucleic acid
(
DNA
) topoisomerases are enzymes which resolve topological problems in eukaryotic
DNA
, and may be involved in cell proliferation. The involvement of
topoisomerase
II in cell proliferation was examined in the human glioma cell line T98G. The growth rate of T98G cells was suppressed by treatment with
topoisomerase
II antisense oligonucleotides dose-dependently, with significant suppression at concentrations greater than of 0.1 mM. The growth rate of T98G treated with control oligonucleotide was suppressed at concentrations greater than 3.0 mM. The activity of
topoisomerase
II in T98G cells treated with 0.5 mM
topoisomerase
II antisense oligonucleotide was one fourth of that in cells treated with control oligonucleotide. When
topoisomerase
II translation was suppressed, the activity of topoisomerase I was increased. These results suggest that de novo synthesis of the
topoisomerase
II protein is required to maintain a normal growth rate in cultured T98G cells. These topoisomerases may be functionally related, and might provide compensatory mechanisms in the case of compromised function.
...
PMID:Inhibition of topoisomerase II alpha subunit de novo synthesis by specific antisense oligonucleotides suppresses human glioma T98G cell growth. 1034 8
