Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:5.99.1.2 (
topoisomerase
)
9,166
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The DNA binding properties of a protein from the lower eukaryote Ustilago maydis have been characterized. Using both filter binding and gel retention assays, we demonstrate that this protein, termed
UBP1
(Ustilago binding protein one), binds preferentially to DNA molecules lacking chain interruptions. The introduction of DNA breaks by a restriction enzyme or a purified nuclease, from Ustilago maydis, causes the dissociation of protein-DNA complexes.
UBP1
stimulates the relaxation of negatively supercoiled DNA, mediated by Ustilago type I
topoisomerase
, through a mechanism most likely involving the association of
UBP1
with the DNA rather than with the
topoisomerase
. The prebinding of
UBP1
to DNA templates, subsequently assembled into minichromosomes, results in the development of a disorganized nucleosomal array. Possible roles for
UBP1
in processes that involve changes in DNA topology, such as chromatin assembly, are discussed.
...
PMID:Characterization of Ustilago maydis DNA binding protein one (UBP1). 839 72
