Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
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Drug
Enzyme
Compound
Query: EC:4.6.1.2 (
guanylate cyclase
)
8,497
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The effects of cyclic GMP on the release of calcium from intracellular stores, induced in murine peritoneal macrophages by either ATP or platelet-activating factor, were studied by microfluorimetry with fura-2. When macrophages were incubated for 10-20 min with 10 microM LY83583, an inhibitor of
guanylate cyclase
, the rise in intracellular calcium induced by agonist application was strongly depressed. This inhibition of the response to platelet-activating factor could be reversed by the addition of 0.1 mM cyclic 8-bromo-GMP. In the presence of cyclic 8-bromo-GMP, the decay of the calcium transient was speeded. Furthermore, when two calcium transients were evoked within 1 min by stimulating the cells with 10 microM ATP, the second calcium transient was more depressed than the first one in the presence of LY83583. These findings are compatible with the hypothesis that cyclic GMP is necessary for the activation of the
calcium pump
of the intracellular stores.
...
PMID:Cyclic-GMP-dependent refilling of calcium stores in macrophages. 164 19
Most of the particle-bound
guanylate cyclase
of pigeon heart muscle, which is the predominant form of the enzyme in this tissue, co-purified with
calcium pump
system of the sarcoplasmic reticular fraction. Specific enzyme activity at 37 degrees C ranged up to 0.67 nmol/mg of protein/min. The K0.5 values for MnCl2, Mn2+ free, and GTP were found to be 0.72, 0.076, and 0.057 mM, respectively. Mg2+ activates and Ca2+ inhibits the enzyme in the presence of Mn2+, whereas carbamylcholine was without effect. The ratio of
guanylate cyclase
to adenylate cyclase activity was found to be 12:1 in the sarcoplasmic reticulum fraction and 0.5:1 in the sarcolemal fraction. Our findings suggest that the major portion of
guanylate cyclase
in pigeon myocardium is not directly activated by the interaction of hormones and neurotransmitters with their receptors in the cell surface membrane.
...
PMID:Subcellular distribution and some properties of particulate guanylate cyclase of pigeon myocardium. 610 52
