Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:4.6.1.2 (guanylate cyclase)
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We have isolated a 3.9-kbp-long cDNA and approximately 93 kbp long genomic DNA encoding a membrane guanylyl cyclase (designated OlGC1) from the medaka fish Oryzias latipes, and determined their nucleotide sequences. The open reading frame for the OlGC1 cDNA predicts a protein of 1,055 amino acids. Phylogenetic analysis indicates OlGC1 to be a medaka fish homolog of mammalian guanylyl cyclase B (GC-B), a member of the natriuretic peptide receptor family. Northern blot analysis demonstrated 3.9 kb OlGC1 transcripts in the eye and brain, while reverse transcription-polymerase chain reaction (RT-PCR) analysis showed OlGC1 transcripts in a number of adult peripheral organs as well as embryos during early stages of development. The OlGC1 gene consists of 22 exons with an exon/intron organization similar to that of the human and rat GC-A genes, except that OlGC1 has several very large introns. The OlGC1 gene has no apparent TATA box or CAAT box in the region 1.2 kbp upstream of the putative transcription initiation point, but several consensus sequences for cis-regulatory elements, including an NF-IL6-binding element, a shear stress responsive element, and multiple GM-CSF-binding elements, are present in that region.
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PMID:Genomic structure and expression of the medaka fish homolog of the mammalian guanylyl cyclase B. 1039 27

Two cDNA clones (OlGC2 and OlGC7) and their genomic DNA clones encoding medaka fish homologs of mammalian natriuretic peptide receptor/membrane guanylyl cyclase A (GC-A) were isolated, and their complete nucleotide sequences were determined. The open reading frame predicts a protein of 1,063 amino acids for OlGC2 cDNA (4,283 bp), and one of 1,055 amino acids for OlGC7 cDNA (3,721 bp), respectively. Northern blot analyses demonstrated 4.7 kb OlGC2 transcripts in the kidney and gill, and 4.0 kb OlGC7 transcripts in the kidney, brain, and ovary, while RNase protection analyses revealed that both genes are expressed in various adult organs. Both the OlGC2 (about 33.0 kbp) and OlGC7 (about 44.3 kbp) genes consist of 22 exons with an exon/intron organization similar to those of the human GC-A gene (about 16.6 kbp) and medaka fish GC-B homolog gene (OlGC1, about 93 kbp). Intron 4 of OlGC2 contains two repeated sequence (RS) clusters, designated as RS1 (about 1 kbp) and RS2 (about 5 kbp), consisting of nucleotide 5'-AGCCTCTGCTCCTCCTTC-3'. In addition, many identical but variably sized nucleotide sequences were found in introns in OlGC1, OlGC2, OlGC6, and OlGC7. The OlGC2 and OlGC7 genes both have no apparent TATA box in the 5' flanking region upstream of the putative transcription initiation point, but several consensus sequences for cis-regulatory elements, including C/EBP, CREB, NF-IL6, and Sp1 and AP-2, NF-IL6, c-Myb, and Sp1 are present in the 5'-flanking region of OlGC2 and OlGC7, respectively.
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PMID:Expression and exon/intron organization of two medaka fish homologs of the mammalian guanylyl cyclase A. 1143 78