Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:4.6.1.1 (adenylate cyclase)
 19,190 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A new bone cell line was established by transfecting normal adult human osteoblast-like (hOB) cells, derived from a 68-year-old woman, with the plasmid pSV3 neo. The plasmid included coding sequences and promotors for the large and small T antigens of the SV40 virus as well as resistance to the antibiotics neomycin and G418. A single antibiotic-resistant colony was located and cloned. Large tumor antigen production in the clonal cell line was confirmed by indirect immunofluorescence study. Treatment with 1,25-dihydroxy-vitamin D3 increased steady-state concentrations of protein and mRNA for osteocalcin and for alkaline phosphatase. Northern blot analyses also demonstrated the presence of mRNAs for alpha(I)-procollagen, osteopontin 1a, transforming growth factor beta, and interleukin-1 beta. The plasma membrane calcium pump and osteonectin were identified by immunocytochemical analysis. These cells produced a matrix that mineralized when beta-glycerophosphate was added to their cultures. As assessed by functional receptor assays, both estrogen and androgen receptors were present and functional, although at low concentrations. Treatment with parathyroid hormone did not stimulate adenylate cyclase activity. Thus, these cells are a well-differentiated, steroid-responsive clonal cell line that closely approximates the phenotype of the mature osteoblast. They should serve as an excellent model for the study of osteoblast biology.
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PMID:Development and characterization of a rapidly proliferating, well-differentiated cell line derived from normal adult human osteoblast-like cells transfected with SV40 large T antigen. 137 29

Two types of proglucagon processing have been evidenced in producing tissues (endocrine pancreas, stomach, intestine, central nervous system) using antibodies recognizing the epitopes unmasked during processing, which takes place at dibasic sites. A first type, leading essentially to glucagon, has been observed in the two former tissues; a second type, leading to peptides (oxyntomodulin and glicentin) containing an additional C-terminal octapeptide, has been shown in the two latter. All peptides are released in plasma and reach their targets: liver, fat... (control of metabolism) for glucagon and gastric mucosa (control of acid secretion) for the octapeptide-bearing peptides. The mode of action of these peptides includes receptors coupled to adenylate cyclase and a processing, at a dibasic site, of the circulating peptides leading to C-terminal fragments which act through non cyclic AMP-dependent mechanisms, such as the control of the plasma membrane calcium pump.
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PMID:[Post-translational maturation of peptides of the glucagon family. Relationship with their mode of action]. 229 22