Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:4.6.1.1 (adenylate cyclase)
 19,190 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

p-Bromophenacyl bromide (BPB) is an alkylating agent which has been used in biochemical studies as an inhibitor of phospholipase A2 activity. We report here that BPB irreversibly inhibited adenylate cyclase activity stimulated by hormones, forskolin, GppNHp, NaF, and cholera toxin. The action of BPB in S49 lymphoma cell membranes (wild type and cyc-) indicates that it can inhibit adenylate cyclase function in the absence of Gs. In the presence of Gs, however, inhibition of adenylate cyclase by BPB was enhanced, suggesting that BPB may covalently modify the catalytic protein on a site involved in activated catalytic functioning or critical to its interaction with Gs and/or additionally on the alpha s protein.
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PMID:Inhibition of adenylate cyclase by p-bromophenacyl bromide. 166 Feb 72

p-Bromophenacyl bromide, a potent alkylating agent inhibitor of phospholipase A2 and adenylate cyclase, potentiates hydrogen peroxide production in human platelets activated with thrombin. The maximal cooperative effect, specific for thrombin as inducer and potentiated by exogenous calcium, was observed at 2 microM BPB. Since it was shown that BPB is also a strong inhibitor of platelet aggregation (IC 50 = 3 +/- 1 microM) it is likely to suppose that the abnormal accumulation of hydrogen peroxide can influence platelet function.
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PMID:p-Bromophenacyl bromide potentiates hydrogen peroxide formation in human platelets challenged by thrombin and inhibits aggregation. 849 15