Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:4.6.1.1 (adenylate cyclase)
 19,190 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The regulation of catabolite repression of beta-galactosidase has been studied in Escherichia coli mutants deleted for the adenyl cyclase gene (cya delta), and thus unable to synthesize cyclic AMP. It has been found that, provided a second mutation occurs either in the crp gene coding for the catabolite gene activator protein (CAP) or in the Lactose region, these mutants exhibit catabolite repression. If the catabolite repression seen in the mutant strains corresponds to the mechanism operating in wild-type cells the results would suggest that the intracellular concentration of cyclic AMP cannot be the unique regulator of catabolite repression.
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PMID:Catabolite repression in Escherichia coli mutants lacking cyclic AMP. 20 9

Our previous evaluation of ruminal and other anaerobic bacteria showed only Prevotella ruminicola D31d produced detectable concentrations of cyclic AMP. In order to investigate the synthesis of this important metabolic regulator, the gene for adenylate cyclase (cya), which produces cyclic AMP, was cloned and expressed in a cyaA mutant of Escherichia coli. The cloned P. ruminicola D31d gene was able to complement the cyaA mutation and permitted fermentation of lactose on MacConkey Lactose agar plates. Analysis of the DNA sequence of the 2.5-kilobase pair insert revealed an open reading frame encoding for a 67-kDa protein. This protein was novel in that no amino acid similarity was observed with other procaryotic or eucaryotic adenylate cyclases in the GenBank database. Production of cyclic AMP in the E. coli clone was confirmed with a radioimmunoassay technique. This is the first example of an adenylate cyclase gene identified from an anaerobic bacterium.
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PMID:Identification of a novel adenylate cyclase in the ruminal anaerobe, Prevotella ruminicola D31d. 968 74