Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:4.6.1.1 (adenylate cyclase)
 19,190 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Luminal brush border and contraluminal basal-lateral segments of the plasma membrane from the same kidney cortex were prepared. The brush border membrane preparation was enriched in trehalase and gamma-glutamyltranspeptidase, whereas the basal-lateral membrane preparation was enriched in (Na+ + K+1)-ATPase. However, the specific activity of (Na+ + K+)-ATPase in brush border membranes also increased relative to that in the crude plasma membrane fraction, suggesting that (Na+ + K+)-ATPase may be an intrinsic constituent of the renal brush border membrane in addition to being prevalent in the basal-lateral membrane. Adenylate cyclase had the same distribution pattern as (Na+ + K+)-ATPase, i.e. higher specific activity in basal-lateral membranes and present in brush border membranes. Adenylate cyclase in both membrane preparations was stimulated by parathyroid hormone, calcitonin, epinephrine, prostaglandins and 5'-guanylylimidodiphosphate. When the agonists were used in combination enhancements were additive. In contrast to the distribution of adenylate cyclase, guanylate cyclase was found in the cytosol and in basal-lateral membranes with a maximal specific activity (NaN3 plus Triton X-100) 10-fold that in brush border membranes. ATP enhanced guanylate cyclase activity only in basal-lateral membranes. It is proposed that guanylate cyclase, in addition to (Na+ + K+)-ATPase, be used as an enzyme "marker" for the renal basal-lateral membrane.
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PMID:Preparation of renal cortex basal-lateral and bursh border membranes. Localization of adenylate cyclase and guanylate cyclase activities. 1 97

In the isolated-perfused frog (Rana pipiens) kidney the question of whether transepithelial transport of Ca2+ is a passive voltage driven process or involves active mechanisms was investigated. With conventional and ion-sensitive microelectrodes transepithelial electrical and electrochemical potential differences were measured. Luminal activities and transepithelial net fluxes of Ca2+ and Cl- were evaluated. Different transepithelial electrical voltages in a wide range (+20 to -4 mV) were generated by "chemical voltage clamping" and the dependence of Ca2+ net fluxes on these voltages investigated. The hormonal control of both Cl- and Ca2+ transport was studied by evaluating the effect of the cell-permeable cAMP analogue, db-cAMP and of the adenylate cyclase stimulator, forskolin. The experiments reveal that: (a) Ca2+ is reabsorbed along the diluting segment of frog kidney. (b) Ca2+ reabsorption is inhibited by furosemide because of the elimination of the transepithelial voltage. (c) There is a direct relationship between transepithelial voltage and Ca2+ reabsorption. (d) Neither Cl- nor Ca2+ reabsorption are affected by db-cAMP or forskolin. We conclude that Ca2+ reabsorption is passive, driven by the lumen-positive transepithelial voltage. It most likely occurs via the paracellular shunt pathway.
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PMID:Ca2+ transport in diluting segment of frog kidney. 282 14

Osmotically balanced solutions of sodium chloride and glucose (5-150 mmol/l) were instilled into rats prepared with two tied intestinal loops (jej-col or il-col). Luminal accumulation or disappearance of Na and glucose after 15 min. was determined, and the parameters of the linear regression lines of net Na flux (y) with initial Na concentration (x) calculated. Control cation and glucose transport were changed by dioctylsulphosuccinate and dodecylsulphate in the way described by Sund & Matheson (1978). Theophylline (10-25 mmol/l) on the other hand did not alter glucose disappearance, and had a distinctly dissimilar effect on net Na transport compared to the surfactants. This could be described as a parallel displacement of the control regression lines to the right, without improvement in correlation. This effect of theophylline was greatest in the ileum, where mean luminal Na concentration corresponding to zero net transport was raised from about 70 mmol/l under control condition to values above 200 mmol/l. The results are consistent with the view that theophylline mainly affects transcellular and the surfactants mainly paracellular sodium transport, and do not support the theory that the effect of dioctylsulphosuccinate on intestinal transport is related to an activation of the mucosal adenylate cyclase/cAMP system, at least not in short term experiments.
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PMID:Net sodium and glucose transport in the jejunum, ileum and colon of anaesthetized rats in response to intraluminal theophylline and anionic surfactants. 627 35