Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:4.6.1.1 (
adenylate cyclase
)
19,190
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The protein kinase C stimulator TPA (12-O-tetradecanoyl phorbol-13-acetate) enhanced the responsiveness of
adenylate cyclase
to IPR (isoproterenol) and PGE1 (prostaglandin E1) in quiescent tsKSV-NRK cells at the nonpermissive 41 degrees C. Reactivating the thermolabile mitogenic/oncogenic
K-ras protein
in tsKSV-NRK cells by dropping the temperature to 36 degrees C also enhanced the responsiveness of
adenylate cyclase
to IPR and PGE1. The enhancement was transient and peaked at 6 hours after the temperature shift. This enhanced responsiveness was specifically due to the reactivated viral
K-ras protein
rather than the temperature shift because the same temperature shift did not affect
adenylate cyclase
responsiveness in uninfected NRK cells, nor was it a result of the mitogenic stimulus since reacting the mitogenic pp60v-src protein in tsASV-NRK cells did not affect
adenylate cyclase
responsiveness. The increased responsiveness of
adenylate cyclase
at 6 hours after the temperature shift was not a result of elevated membrane-associated PKC activity. However, the reactivated viral
K-ras protein
strongly increased the stimulability of membrane-associated PKC by TPA and it further increased TPA's ability to enhance the responsiveness of
adenylate cyclase
to IPR and PGE1. Thus, a viral
K-ras protein
and membrane-associated protein kinase C can cooperate to increase the responsiveness of
adenylate cyclase
to agonists.
...
PMID:Protein kinase C and a viral K-RAS protein cooperatively enhance the response of adenylate cyclase to stimulators. 255 Apr 70
