Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:4.2.3.23 (
GAS
)
957
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
It has been demonstrated that interferon-gamma (IFN-gamma) and
interleukin-10
(
IL-10
) have various reverse effects on macrophages; however, the molecular mechanism of this difference has not been fully understood. In this study, we analyzed the binding activity of
IL-10
- and IFN-gamma-activated STAT molecules to two kinds of
GAS
-motif sequences.
IL-10
-activated STAT1 could bind to the
GAS
-motif sequence in the promoter region of the Fcgamma receptor, but not to that in the promoter region of the COX-2 gene, whereas IFN-gamma-activated STAT1 and STAT5 could bind to both sequences.
IL-10
inhibited IFN-gamma-induced STAT activation without newly synthesized protein. We further demonstrated that aspirin, but not dexamethasone, suppressed IFN-gamma-induced STAT activation. Taken together, these results suggest that
IL-10
-activated STAT1 has a specificity in binding to the
GAS
-motif sequences, whereas IFN-gamma-activated STAT1 and STAT5 have a broader spectrum in binding to the
GAS
-motif sequences. This may explain the difference between
IL-10
and IFN-gamma in biological activity, and the inhibitory effect of
IL-10
on IFN-gamma activities.
...
PMID:Selective DNA-binding activity of interleukin-10-stimulated STAT molecules in human monocytes. 1043 70
