Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:4.2.3.23 (GAS)
 957 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Streptococcus pyogenes (group A streptococcus, GAS) is a Gram-positive bacterial pathogen responsible for a wide variety of diseases. To date, GAS vaccine development has focused primarily on the M-protein. The M-protein is highly variable at the amino (N)-terminus (determining serotype) but is conserved at the carboxyl (C)-terminus. Previously a 29 amino acid peptide (named J14) from the conserved region of the M-protein was identified as a potential vaccine candidate. J14 was capable of eliciting protective antibodies that recognized many GAS serotypes when co-administered with immuno-stimulants. This minimal epitope however showed no immunogenicity when administered alone. In an attempt overcome this immunological non-responsiveness, we developed a self-adjuvanting vaccine candidate composed of three components: the B-cell epitope (J14), a universal helper T-cell epitope (P25) and a lipid moiety consisting of lipoamino acids (Laas) which target Toll-like receptor 2 (TLR2). Immunological evaluation in B10.BR (H-2k) mice demonstrated that the epitope attachment to the point of lipid moiety, and the length of the Laa alkyl chain have a profound effect on vaccine immunogenicity after intranasal administration. It was demonstrated that a vaccine featuring C-terminal lipid moiety containing alkyl chains of 16 carbons, with P25 located at the N-terminus, and J14 attached to the side chain of a central lysine residue was capable of inducing optimal antibody response. These findings have considerable relevance to the development of a broad spectrum J14-based GAS vaccine and in particular provided a rational basis for peptide vaccine design based on this self-adjuvanting lipopeptide technology.
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PMID:Immunological evaluation of lipopeptide group A streptococcus (GAS) vaccine: structure-activity relationship. 2225 11

In the present study, Terminalia bellirica methyl ester (TBME)/biodiesel was formed using potassium hydroxide as catalyst. Methyl ester fuel properties were resolved as per ASTM standard. The attribute of compression ignition (CI) engine such as combustion, performance, and released emission qualities of samples, such as diesel and TBME blends (B10, B20, B30, B50, and B100), were deliberated on a single-cylinder CI engine. By varying the load to 0%, 25%, 50%, 75%, and 100%, test data were measured. Using AVL DI GAS 444N (five gas) analyzer dissimilarity in the engine emissions were documented. The results of biodiesel test blends show the similar combustion and performance to diesel curves with minor decrement. However, CO and HC emission diminish when engine fueled with TBME test blends whereas NOx amplifies.
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PMID:Terminalia bellirica: a new biodiesel for diesel engine: a comparative experimental investigation. 3178 62