Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:4.2.2.7 (
heparinase
)
1,270
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Human cytomegalovirus (HCMV) is known to down-regulate the expression of human leukocyte antigen (HLA) class I, the process of which involves a subset of virus genes. Infection of human foreskin fibroblast (HFF) cells with UV-inactivated HCMV (UV-HCMV), however, resulted in an increase in
HLA class I
presentation on the cell surface in the absence of HCMV gene expression. Heparin, which inhibits the interaction of virus particles with cell surface heparan sulfate proteoglycans (HSPGs), blocked the effect of UV-HCMV on
HLA class I
expression. Pretreatment of cells with
heparinase
I decreased in a dose-dependent manner the effect of UV-HCMV on
HLA class I
expression enhancement. Sodium chlorate, which is known to inhibit the sulfation of HSPGs, gave a similar result. Pretreatment of UV-HCMV with trypsin or monoclonal antibody reactive with the envelope glycoprotein gB reduced the increase in
HLA class I
expression on the HFF cell surface by UV-HCMV. RT-PCR analysis demonstrated that the increase in
HLA class I
presentation on the HFF cell surface was due to an increase in
HLA class I
transcription. Thus, binding of HCMV particles to cell surface HSPGs appears to be required for the stimulation of
HLA class I
expression. It is also possible that virus entry, in addition to binding to HSPGs, may be involved in the stimulation of
HLA class I
expression, since the UV-HCMV entered the cells and all treatments to block virus binding to HSPGs would necessarily prevent virus entry.
...
PMID:Human cytomegalovirus binding to heparan sulfate proteoglycans on the cell surface and/or entry stimulates the expression of human leukocyte antigen class I. 1156 34
