Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:4.2.2.7 (heparinase)
 1,270 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The effects of several neurohumoral agents and serine proteases on glycoconjugate release from hamster tracheal organ cultures were assessed. The beta-adrenergic agonist isoproterenol inhibited glycoconjugate release, and its effect was abolished by the specific beta-blocking agent propranolol. A cholinergic agonist, pilocarpine, marginally increased glycoconjugate release, and its effect was abolished by the antagonist atropine. Human neutrophil elastase and porcine pancreatic trypsin consistently increased glycoconjugate release by 1.8 to 2.8-fold. When the proteases were inactivated, they were no longer effective in stimulating glycoconjugate release. Histologic and electron microscopic analysis of the protease-treated organ cultures revealed no discernible toxic reaction. In addition, organ cultures prelabeled with chromium 51 did not release an increased amount of radioactivity when treated with the proteases. Biochemical analysis of the glycoconjugates released into the culture medium showed them to be of high molecular weight (90% eluted in the void volume of a Sepharose 6B column) and to be resistant to digestion with hyaluronidase and heparinase, properties consistent with mucous glycoproteins. The mechanism of protease-induced glycoconjugate release is unknown. We speculate that stimulation of airway secretory cells by serine proteases of neutrophilic or other inflammatory cell origin may play a role in the increased airway secretion that is characteristic of acute tracheobronchitis.
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PMID:Serine proteases stimulate mucous glycoprotein release from hamster tracheal ring organ culture. 287 41

A collection of 50 clinical isolates of Bacteroides was examined for plasmid deoxyribonucleic acid content. An attempt was then made to correlate the presence of plasmids with a specific phenotypic property. Of the 20 Bacteroides which contained plasmids, 18 were found to harbour plasmids of less than or equal to 9.8 megadaltons. The most common plasmid had a molecular weight of 4.8 megadaltons and was found in 9 strains. Most strains had multiple plasmid bands. All strains were examined for resistance to penicillin, cefoxitin, erythromycin, tetracycline, sulphamethoxazole, clindamycin, chloramphenicol, arsenate, silver, cadmium, mercury, chromium, lead, nickel and cobalt, and for the production of beta-lactamase, heparinase, deoxyribonuclease, haemolysins and bacteriocins. Using a Chi-squared analysis, there was no statistically significant correlation between any of these phenotypic traits and the presence of plasmids, except bacteriocin production. A total of 15 out of 20 (75%) of plasmid-containing strains produced bacteriocins while only 10 out of 30 (33%) of plasmid-free strains were capable of bacteriocin production (chi 2, p less than 0.005). Attempts to transfer or cure resistance to antibiotics and heavy metals or bacteriocin production were not successful.
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PMID:Physiological properties and plasmid content of Bacteroides spp. 653 4