Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:4.2.2.7 (
heparinase
)
1,270
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Identification with specific heparan sulfate-lyases, heparitinase I and
heparinase
of the constitutional unsaturated disaccharide (delta Di-
SHS
) derived from heparan sulfate (HS) isomers and heparin was achieved using high-performance liquid chromatography (HPLC) with a sulfonated styrene-divinylbenzene copolymer. Eight delta Di-
SHS
products derived from HS isomers were identified. Enzymatic digestion with heparitinase I and
heparinase
converts heterogeneous sulfated HS isomers and heparin into different delta Di-
SHS
. The practical application of these enzymes was examined using specific enzymes and HPLC. In a patient with Hurler syndrome, eight individual delta i-
SHS
were identified in urinary HS isomers.
...
PMID:High-performance liquid chromatographic identification of eight constitutional disaccharides from heparan sulfate isomers digested with heparitinases. 749 82
