Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:4.2.2.7 (
heparinase
)
1,270
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Heparinase I has important applications in the fields of biomedicine and pharmaceuticals. The
heparinase
I gene (HpaI) from Flavobacterium heparinum was cloned and overexpressed in Pichia pastoris GS115, and the conditions for the
heparinase
I production were optimized by
RSM
. PCR analysis indicated that HpaI was integrated into the P. pastoris GS115 genome. The concentrations of key factors that affected the
heparinase
I activity were optimized, and were as follows: oleic acid, 0.07%, liquid volume in flask, 34.3 ml/L, and methanol, 0.96%. Under the optimal conditions, the activity of
heparinase
I was up to 323 U/L in shake flask. A maximal
heparinase
I activity of 398.5 U/L from the transformant 2 was achieved in a 5L fermentor. This study demonstrates the overproduction of
heparinase
I by recombinant P. pastoris.
...
PMID:Expression of HpaI in Pichia pastoris and optimization of conditions for the heparinase I production. 2472 Oct 72
