Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:4.2.2.10 (
PNL
)
341
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Fast production and purification of alpha-(1,4)-oligogalacturonides was investigated using a new enzymatic reactor composed of a monolithic matrix. Pectin lyase from Aspergillus japonicus (Sigma) was immobilized on
CIM
-disk epoxy monolith. Studies were performed on free
pectin lyase
and immobilized
pectin lyase
to compare the optimum temperature, optimum pH, and thermal stability. It was determined that optimum temperature for free
pectin lyase
and immobilized
pectin lyase
on monolithic support is 30 degrees C, and optimum pH is 5. Monolithic
CIM
-disk chromatography is one of the fastest liquid chromatographic method used for separation and purification of biomolecules due to high mass transfer rate. In this context, online one step production and purification of oligogalacturonides was investigated associating
CIM
-disk
pectin lyase
and
CIM
-disk DEAE. This efficient enzymatic bioreactor production of uronic oligosaccharides from polygalacturonic acid (PGA) constitutes an original fast process to generate bioactive oligouronides.
...
PMID:New monolithic enzymatic micro-reactor for the fast production and purification of oligogalacturonides. 1787 Jun 76
