Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:4.2.2.10 (
PNL
)
341
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
A
pectin lyase
gene (pnl) of Pseudomonas marginalis was cloned and overexpressed in Escherichia coli BL21(DE3). The pnl gene was amplified by PCR, inserted into pET29c with a six-His tag and the overproduced active enzyme was purified almost to homogeneity using a Ni(2+)-nitrilotriacetate-agarose column. The purified
pectin lyase
(
PNL
;
EC 4.2.2.10
, family 1) is inhibited by
NAD+
(at concentrations above 0.25 mM), NADH or dithiothreitol. Evidence for the existence of a heat-labile protein inhibitor of
PNL
is also reported. The DNA-binding ability of
PNL
was demonstrated by DNA-retardation experiments. The partially purified enzyme was incubated with plasmid DNA and the complex was shifted to a higher molecular mass. Analysis of the electroeluted proteins from the protein-DNA complex revealed that one of the electroeluted protein bands was
PNL
. Antibodies against the overexpressed
PNL
were also prepared and partially purified.
...
PMID:Overexpression of the pectin lyase gene of Pseudomonas marginalis in Escherichia coli and purification of the active enzyme. 1263 Sep 8
