Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:4.2.2.10 (PNL)
 341 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Tissue maceration was generally elucidated by the action of endo-polygalacturonase and endo-pectate or -pectin lyase (endo-PAL or -PNL). In a process of screening of Erwinia and Pseudomonas strains for enzymatic pulping of pectocellulosic bast fibers, it was found that their PAL productivity was not completely related with defibration activity, i.e., the fact that an E.chrysanthemi strain showed high PAL productivity but possessed rather low defibration activity. Moreover, defibration activity was parallel to the amount of neutral sugars released during pulping. Based on these fact, the maceration or enzymatic pulping of basts was estimated to proceed not only by cleavage of interfiber bonding cause by PAL action but also another factors. Among three possibilities proposed on the maceration mechanism of basts, it was elucidated by a concerted action of PAL and PNL with an aid of xylanase. In addition, a quantitative determinative method of maceration activity toward basts was also presented.
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PMID:Approach to maceration mechanism in enzymatic pulping of bast fibers by alkalophilic pectinolytic enzymes produced by Erwinia species. 1454 40

The effect of various parameters such as pH, agitation and aeration was studied for maximum production of pectin lyase (PL) and pectate lyase (PGL) by a novel yeast strain Debaryomyces nepalensis in bioreactor. The optimal levels of pH, aeration and agitation rate was found to be 7.0, 300rpm and 1vvm, respectively. Under these conditions, D. nepalensis produced 14,200U/L of PL and 12,000U/L of PGL corresponding to a productivity of 600U/Lh and 500U/Lh of PL and PGL, respectively. Fed-batch production was studied by feeding inducer (lemon peel), carbon source (galactose) individually and in combination at 12h of growth for enhanced production of PL and PGL. Combined feeding of inducer and carbon source at 12h was found to be the best strategy for enhanced production of PL and PGL. Under these conditions, production of PL and PGL increased to 23,300U/L and 22,400U/L, respectively which corresponded to a productivity of 728U/Lh of PL and 700U/Lh of PGL, respectively. The production was increased by 1.6- and 1.8-fold and productivity by 1.2- and 1.4-fold for PL and PGL, respectively when compared to batch culture.
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PMID:Batch and fed batch production of pectin lyase and pectate lyase by novel strain Debaryomyces nepalensis in bioreactor. 1739 59