Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:4.2.2.10 (
PNL
)
341
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
An indigenously isolated fungal strain identified as Aspergillus terricola with assigned fungal strain number MTCC 7588 has been used as source for
pectin lyase
production. The extracellular
pectin lyase
was purified to homogeneity from the culture filtrate of A. terricola by ion exchange and gel filtration chromatography. The determined molecular weight was 35 +/- 01 kDa. The K(m) and k(cat) (turnover) values of the purified enzyme at 37 degrees C using citrus pectin as the substrate were found to be 1.0 mg/ml and 110.0 s(-1), respectively. The pH and temperature optima of the enzyme were 8.0 and 50 degrees C, respectively. The retting ability of the purified
pectin lyase
for natural fibers viz.
Cannabis
sativa and Linum usitatissimum has been demonstrated for the first time.
...
PMID:Purification and characterization of pectin lyase produced by Aspergillus terricola and its application in retting of natural fibers. 1908 48
The importance of various parameters such as sugarcane juice concentration, pH of the medium, and effects of different solid supports for maximum secretion of
pectin lyase
from Penicillium citrinum MTCC 8897 has been studied. The enzyme was purified to homogeneity by Sephadex G-100 and DEAE-cellulose chromatography. The molecular mass determined by SDS-PAGE was 31 kDa. The K(m) and k(cat) values were found to be 1 mg/ml and 76 sec(-1), respectively. The optimum pH of the purified
pectin lyase
was 9.0, though it retains activity in the pH 9.0-12.0 range when exposed for 24 h. The optimum temperature was 50 degrees C, and the
pectin lyase
was found to be completely stable up to 40 degrees C when exposed for 1 h. The purified
pectin lyase
was found efficient in retting of Linum usitatissimum,
Cannabis
sativa, and Crotalaria juncea.
...
PMID:Purification and characterization of pectin lyase secreted by Penicillium citrinum. 1974 2
