Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:4.1.99.3 (
PRE
)
1,923
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Cis-acting regions in the 5'-flank of the mouse
interleukin 5
(
IL-5
) gene involved in the specific and inducible regulation of
IL-5
transcription in an untransformed mouse T cell clone, D10.G4.1, have been identified. Transient transfection assays with a series of deletion
IL-5
promoter reporter constructs indicate that multiple regulatory elements in the 5'-flanking region of the
IL-5
promoter play a role in regulating
IL-5
transcription in Th2 cells. Negatively acting elements, NRE I and NRE II, map to the regions between positions -431 and -392 and positions -300 and -261. A positive regulatory element has been mapped to the region between positions -224 and -81. The activity of these elements is dependent on activation of the cells. A 40-bp sequence within this region, termed the
IL-5
PRE
, has been shown to bind at least two specific nuclear protein complexes from unstimulated and stimulated D10.G4.1 cells. An additional protein complex specific for this site has been identified in nuclear fractions from cells stimulated in the presence of the protein synthesis inhibitor, cycloheximide. Proteins that bind to these elements are likely to be important inducible and specific factors essential for control of
IL-5
transcription in response to T cell receptor-mediated signaling events.
...
PMID:Transcription of the murine interleukin 5 gene is regulated by multiple promoter elements. 765 35
