Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:4.1.99.3 (PRE)
 1,923 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The high mobility group (HMG) I proteins are small, non-histone chromosomal proteins that promote gene activation during development and within rapidly dividing cells. They do so by facilitating enhanceosome formation on inducible genes, via both protein/DNA and protein/protein interactions. The HMG I-C gene is tightly regulated, normally being expressed exclusively during embryonic development. However, HMG I-C expression is also observed frequently in a number of tumor types, and this expression has been shown to contribute to the malignant transformation process. With the aim of dissecting pathways that lead to aberrant expression of HMG I-C in tumor cells, we have analyzed HMG I-C gene regulation in the human hepatoma cell line PLC/PRF/5. One of the two HMG I-C transcripts detected in this cell line originates from a novel downstream initiation site at nucleotide -161 relative to the first methionine. Transcription from the downstream initiation site is mediated by a PRE located between nt -222 and -217. We show here that the Sp1 and Sp3 transcription factors interact with the PRE and transactivate the HMG I-C promoter in a cooperative fashion. This study provides the first characterization of this downstream HMG I-C promoter.
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PMID:A novel downstream positive regulatory element mediating transcription of the human high mobility group (HMG) I-C gene. 1047 23

DNA photolyase recognizes ultraviolet-damaged DNA and breaks improperly formed covalent bonds within the cyclobutane pyrimidine dimer by a light-activated electron transfer reaction between the flavin adenine dinucleotide, the electron donor, and cyclobutane pyrimidine dimer, the electron acceptor. Theoretical analysis of the electron-tunneling pathways of the DNA photolyase derived from Anacystis nidulans can reveal the active role of the protein environment in the electron transfer reaction. Here, we report the unexpectedly important role of the single methionine residue, Met-353, where busy trafficking of electron-tunneling currents is observed. The amino acid conservation pattern of Met-353 in the homologous sequences perfectly correlates with experimentally verified annotation as photolyases. The bioinformatics sequence analysis also suggests that the residue plays a pivotal role in biological function. Consistent findings from different disciplines of computational biology strongly suggest the pivotal role of Met-353 in the biological function of DNA photolyase.
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PMID:Discrimination of class I cyclobutane pyrimidine dimer photolyase from blue light photoreceptors by single methionine residue. 1805 35

Recently, genome wide association studies showed that there is a strong association between abacavir-induced serious, idiosyncratic, adverse drug reactions (ADRs) and human leukocyte antigen-B*5701 (HLA-B*5701). Studies also found that abacavir-induced ADRs were seldom observed in patients carrying the HLA-B*5801 subtype. HLA-B*5801 of the same serotype (B17) as B*5701 differs by only 4 amino acids from B*5701. It is believed that because of these sequence differences, HLA-B*5801 cannot bind the specific peptides which are required for HLA-B*5701 to stimulate the T cell immune response. Thus, the difference in peptide binding profiles between HLA-B*5701 and B*5801 is an important clue for exploring the mechanisms of abacavir-induced ADRs. VHSE (principal component score vector of hydrophobic, steric, and electronic properties), a set of amino acid structural descriptors, was employed to establish QSAR models of peptide-binding affinities of HLA-B*5701 and B*5801. Optimal linear SVM (support vector machine) models with high predictive capabilities were obtained for both B*5701 and B*5801. The R(2) (coefficient of determination), Q(2) (cross-validated R(2)), and R(PRE)(2) (R(2) of test set) of two optimal models were 0.7530, 0.7037, 0.6153 (B*5701) and 0.6074, 0.5966, 0.5762 (B*5801), respectively. For B*5701 and B*5801, the mutations in positions 45 (MET-THR) and 46 (ALA-GLU) have little influence on the selection specificity of the P2 position of the bound peptide. However, the mutation in position 97 (VAL-ARG) greatly influences the selection specificity of the P7 position. HLA-B*5701 prefers the bulky and positively charged amino acids at the P7 position. In contrast, HLA-B*5801 prefers the non-polar hydrophobic amino acids at the P7 position while positively charged amino acids are unfavored.
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PMID:Peptide binding specificities of HLA-B*5701 and B*5801. 2301 31

There is a worldwide interest in how lockdown affects physical activity (PA) during the COVID-19 pandemic. Although it has been shown that the mandated stay-at-home restrictions and self-isolation measures applied in different countries were accosiated with a reduction in physical exercise and activity, such results derive from studying only specific periods of lockdown. However, in order for this hypothesis to be tested, consecutive lockdown periods need to be examined separately. In this study we focus on PA change in Greek adults over time, during each of the last four weeks of lockdown in Greece. The web-based Active-Q questionnaire (see Supplementary file 1_Active-Q) was used to collect data prior to the COVID-19 crisis (PRE condition) and during lockdown measures (POST condition). The period of data collection (5 April to 3 May 2020) was divided into four phases (Ph-I, Ph-II, Ph-III, Ph-V), corresponding to the 3rd, 4th, 5th and 6th lockdown week respectively (out of a six-week total lockdown). There were four independent groups of respondents (G-I, G-II, G-III, G-V) who reported their age, weight, height and usual PA habits. Energy expenditure (EE) was calculated (MET-min/week; see Supplementary file 2_Data) in four main different domains (daily occupation activities, means of transportation to and from daily occupation, leisure time and regular sporting activities; see Supplementary file 3_Corresponding MET values). Each group's dataset corresponded to one of the aforementioned phases (G-I to Ph-I, G-II to Ph-II, and so on). Overall PA change (from PRE to POST condition) ranged from -21.50% in G-I (Ph-I) to -5.03 in G-V (Ph-V); PA change in male subgroups ranged from -26.10% in Ph-I to -13.64 in Ph-V; in female subgroups it ranged from -17.42% in Ph-I to -1.39 in Ph-V. Although the decline in overall PA is evident in all groups during each lockdown phase (p<0.05), the combination of our data demonstrates that towards the end of lockdown this decline showed a gradual decreasing tendency.
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PMID:Evolution of changes in physical activity over lockdown time: Physical activity datasets of four independent adult sample groups corresponding to each of the last four of the six COVID-19 lockdown weeks in Greece. 3293 72