Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:4.1.99.3 (PRE)
 1,923 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The purpose of this study was to evaluate changes in systolic arterial blood pressure (SABP) immediately after collection of blood for transfusion in retired racing Greyhounds. We prospectively evaluated 19 blood donor Greyhounds before and after the collection of a unit (450 mL) of blood. The SABP was measured with Doppler in the right forearm after the dogs had been in the blood collection room for a few minutes (PRE-FLOOR) and again 5-10 minutes after the dogs were placed on the table where they would be bled (PRE-TABLE). A total of 3-5 minutes after completing the blood collection, the SABP was measured again while the dogs were still in lateral recumbency on the table (POST-TABLE) and once more 60-90 minutes later, when the dogs were on the floor after completing the donation (POST-FLOOR). All dogs were monitored for clinical signs of hypotension, including depression, weakness, collapse, and pallor, for a minimum of 2 hours after donation. There was a significant difference in SABP for the group between PRE-FLOOR and POST-TABLE (P = .02) and between PRE-TABLE and POST-TABLE determinations (P = .01). There were no significant differences for any of the other time points; there were no adverse events. Therefore, we conclude that the collection of 450 mL of blood from normal Greyhounds results in a short-lived yet significant decrease in SABP, but the likelihood of adverse events is negligible.
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PMID:Effects of blood donation on arterial blood pressure in retired racing Greyhounds. 1635 13

In order to characterize the mechanical effects of thoracic percussion per se in lung parenchyma, we analyzed respiratory impedance parameters by impulse oscillometry (12 healthy subjects) and lung mechanics by the least square method (6 healthy subjects) before (PRE-TP) and after (POS-TP) thoracic percussion, and after a deep-breath-maneuver (POS-DB). Pulmonary resistance was similar among PRE-TP, POS-TP and POS-DB while pulmonary dynamic compliance showed a significant reduction after TP (mean +/- SEM: from 0.15 +/- 0.018 L/cmH(2)O to 0.12 +/- 0.016 L/cmH(2)O; P = 0.001), returning to basal values (mean +/- SEM: 0.15 +/- 0.021; P = 0.004) after DB. Reactance parameters (AX and f (0)) evaluated by oscillometry increased significantly after TP, returning to previous values after DB. Total impedance (Z5) and respiratory system resistance at 5 Hz (R5) did not differ significantly among experimental conditions. Our data strongly suggest that thoracic percussion can promote changes in respiratory mechanics compatible with lung collapse and/or pneumoconstriction, which are, however, reversible by deep inspirations.
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PMID:Thoracic percussion yields reversible mechanical changes in healthy subjects. 1858 97

Phosphoinositides (PIs) are lipid components of cell membranes that regulate a wide variety of cellular functions. Here we exploited the blue light-induced dimerization between two plant proteins, cryptochrome 2 (CRY2) and the transcription factor CIBN, to control plasma membrane PI levels rapidly, locally, and reversibly. The inositol 5-phosphatase domain of OCRL (5-ptase(OCRL)), which acts on PI(4,5)P(2) and PI(3,4,5)P(3), was fused to the photolyase homology region domain of CRY2, and the CRY2-binding domain, CIBN, was fused to plasma membrane-targeting motifs. Blue-light illumination (458-488 nm) of mammalian cells expressing these constructs resulted in nearly instantaneous recruitment of 5-ptase(OCRL) to the plasma membrane, where it caused rapid (within seconds) and reversible (within minutes) dephosphorylation of its targets as revealed by diverse cellular assays: dissociation of PI(4,5)P(2) and PI(3,4,5)P(3) biosensors, disappearance of endocytic clathrin-coated pits, nearly complete inhibition of KCNQ2/3 channel currents, and loss of membrane ruffling. Focal illumination resulted in local and transient 5-ptase(OCRL) recruitment and PI(4,5)P(2) dephosphorylation, causing not only local collapse and retraction of the cell edge or process but also compensatory accumulation of the PI(4,5)P(2) biosensor and membrane ruffling at the opposite side of the cells. Using the same approach for the recruitment of PI3K, local PI(3,4,5)P(3) synthesis and membrane ruffling could be induced, with corresponding loss of ruffling distally to the illuminated region. This technique provides a powerful tool for dissecting with high spatial-temporal kinetics the cellular functions of various PIs and reversibly controlling the functions of downstream effectors of these signaling lipids.
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PMID:Optogenetic control of phosphoinositide metabolism. 2284 41