Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:4.1.99.3 (PRE)
 1,923 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The low survival of microbial pest control agents exposed to UV is the major environmental factor limiting their effectiveness. Using gene disruption we demonstrated that the insect pathogenic fungus Metarhizium robertsii uses photolyases to remove UV-induced cyclobutane pyrimidine dimers (CPD) and pyrimidine (6-4) photoproducts [(6-4)PPs] from its DNA. However, this photorepair is insufficient to fix CPD lesions and prevent the loss of viability caused by seven hours of solar radiation. Expression of a highly efficient archaeal (Halobacterium salinarum) CPD photolyase increased photorepair >30-fold in both M. robertsii and Beauveria bassiana. Consequently, transgenic strains were much more resistant to sunlight and retained virulence against the malaria vector Anopheles gambiae. In the field this will translate into much more efficient pest control over a longer time period. Conversely, our data shows that deleting native photolyase genes will strictly contain M. robertsii to areas protected from sunlight, alleviating safety concerns that transgenic hypervirulent Metarhizium spp will spread from mosquito traps or houses. The precision and malleability of the native and transgenic photolyases allows design of multiple pathogens with different strategies based on the environments in which they will be used.
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PMID:Enhanced UV resistance and improved killing of malaria mosquitoes by photolyase transgenic entomopathogenic fungi. 2291 89

Immunity to Plasmodium falciparum is contributed by many known and unknown factors. Lack of effective vaccine and rise in drug-resistant Plasmodium parasites leads to the major challenge in controlling the parasite. Determination of surface or secreted proteins which are essential for the survival of the pathogen is a very important step in finding potential vaccine candidates. Despite the discovery of several vaccine candidates against Plasmodium over the last decades, an effective vaccine is not available. The present study hypothesized that the PRE-binding protein (PREBP) could be a potential vaccine candidate against malaria pathogenesis. PREBP is highly conserved among the class Aconoidasida and exhibit high antigenicity and accessibility as a secretory protein. The present hypothesis was tested by employing in-silico translational genomics wherein the antigenicity, localization and the conservancy were determined.
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PMID:PRE-binding protein of Plasmodium falciparum is a potential candidate for vaccine design and development: An in silico evaluation of the hypothesis. 3090 38