Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
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Target Concepts:
Gene/Protein
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Query: EC:4.1.2.13 (
aldolase
)
3,461
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The focus of this study was the mechanism of starch accumulation in
Chlamydomonas reinhardtii
high-starch mutants. Three
C
.
reinhardtii
mutants showing high-starch content were generated using gamma irradiation. When grown under nitrogen-deficient conditions, these mutants had more than twice as much starch than a wild-type control. The mechanism of starch over-accumulation in these mutants was studied with comparative transcriptome analysis. In all mutants, induction of
phosphoglucomutase 1
(
PGM1
) expression was detected;
PGM1
catalyzes the inter-conversion of glucose 1-phosphate and glucose 6-phosphate in both starch biosynthetic and glycolytic pathway. Interestingly, transcript levels of
phosphoglucose isomerase 1
(
PGI1
),
fructose 1,6-bisphosphate
aldolase
1
and
2
(
FBA1
and
FBA2
) were down-regulated in all mutants; PGI1, FBA1, and FBA2 act on downstream of glucose 6-phosphate conversion in glycolytic pathway. Therefore, down-regulations of
PGI1, FBA1
, and
FBA2
may lead to accumulation of upstream metabolites, notably glucose 6-phosphate, resulting in induction of
PGM1
expression through feed-forward regulation and that
PGM1
overexpression caused starch over-accumulation in mutants. These results suggest that PGI1, FBA1, FBA2, and
PGM1
correlate with each other in terms of coordinated transcriptional regulation and play central roles for starch over-accumulation in
C
.
reinhardtii
.
...
PMID:The Mechanism of Starch Over-Accumulation in
Chlamydomonas reinhardtii
High-Starch Mutants Identified by Comparative Transcriptome Analysis. 2858 57
