Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
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Enzyme
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Query: EC:4.1.2.13 (
aldolase
)
3,461
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Recently, we identified an alginate-assimilating gene cluster in the genome of
Flavobacterium
sp. strain UMI-01, a member of
Bacteroidetes
.
Alginate
lyase genes and a 4-deoxy-l-erythro-5-hexoseulose uronic acid (DEH) reductase gene in the cluster have already been characterized; however, 2-keto-3-deoxy-d-gluconate (KDG) kinase and 2-keto-3-deoxy-6-phosphogluconate (KDPG)
aldolase
genes, i.e.,
flkin
and
flald
, still remained uncharacterized. The amino acid sequences deduced from
flkin
and
flald
showed low identities with those of corresponding enzymes of
Saccharophagus degradans
2-40
T
, a member of
Proteobacteria
(Kim et al., Process Biochem., 2016). This led us to consider that the DEH-assimilating enzymes of
Bacteroidetes
species are somewhat deviated from those of
Proteobacteria
species. Thus, in the present study, we first assessed the characteristics in the primary structures of KDG kinase and KDG
aldolase
of the strain UMI-01, and then investigated the enzymatic properties of recombinant enzymes, recFlKin and recFlAld, expressed by an
Escherichia coli
expression system. Multiple-sequence alignment among KDG kinases and KDG aldolases from several
Proteobacteria
and
Bacteroidetes
species indicated that the strain UMI-01 enzymes showed considerably low sequence identities (15%-25%) with the
Proteobacteria
enzymes, while they showed relatively high identities (47%-68%) with the
Bacteroidetes
enzymes. Phylogenetic analyses for these enzymes indicated the distant relationship between the
Proteobacteria
enzymes and the
Bacteroidetes
enzymes, i.e., they formed distinct clusters in the phylogenetic tree. recFlKin and recFlAld produced with the genes
flkin
and
flald,
respectively, were confirmed to show KDG kinase and KDPG aldolase activities. Namely, recFlKin produced 1.7 mM KDPG in a reaction mixture containing 2.5 mM KDG and 2.5 mM ATP in a 90-min reaction, while recFlAld produced 1.2 mM pyruvate in the reaction mixture containing 5 mM KDPG at the equilibrium state. An in vitro alginate-metabolizing system constructed from recFlKin, recFlAld, and previously reported alginate lyases and DEH reductase of the strain UMI-01 could convert alginate to pyruvate and glyceraldehyde-3-phosphate with an efficiency of 38%.
...
PMID:Identification of 2-keto-3-deoxy-d-Gluconate Kinase and 2-keto-3-deoxy-d-Phosphogluconate Aldolase in an Alginate-Assimilating Bacterium, Flavobacterium sp. Strain UMI-01. 2821 76
