Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:4.1.2.13 (aldolase)
 3,461 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

1. The C(14)O(2) production by Arbacia eggs and embryos from glucose-1-C(14), glucose-2-C(14), and glucose-6-C(14) has been measured without and with dinitrocresol in the incubation medium. In the absence of the dinitrocresol, the C(14)O(2) production from glucose-1-C(14) is more rapid than from glucose-2-C(14) and much more rapid than from glucose-6-C(14); this, together with previous findings, indicates that glucose is utilized in Arbacia eggs predominantly via the TPN shunt rather than via the aldolase step of the glycolytic pathway. In the presence of the dinitrocresol, C(14)O(2) from glucose-6-C(14) approaches that from glucose-1-C(14), indicating that, in the presence of this reagent, glucose utilization is diverted from the shunt to the glycolytic pathway. 2. Incorporation of C(14) from glucose labelled in the 1-, 2-, or 6- positions into other metabolic products of the eggs and embryos is also inhibited by dinitrocresol, particularly incorporation into the acid-insoluble fraction containing nucleoproteins.
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PMID:Alteration by dinitrocresol of pathways for glucose oxidation in eggs of arbacia punctulata. 1335 35

The inhibition of photosynthetic CO(2) fixation by O(2), commonly referred to as the Warburg effect, was examined in isolated intact spinach (Spinacia oleracea) chloroplasts. The major characteristics of this effect in isolated chloroplasts are rapid reversibility when O(2) is replaced by N(2), an increased inhibition by O(2) at low concentrations of CO(2) and a decreased effect of O(2) with increased concentrations of CO(2).Both the DPN- and TPN-linked glyceraldehyde 3-phosphate dehydrogenases but not aldolase were inhibited by O(2). The photoreduction of TPN measured in fragmented chloroplast preparations was similar in N(2) and O(2) down to a concentration of 5 micromolar TPN. The effect of 100% O(2) on (14)CO(2) assimilation was overcome completely by fructose 1,6-diphosphate and by ribose 5-phosphate but not by ascorbate, cysteine, dithiothreitol and reduced lipoate. Glycolate became the major photosynthetic product at high partial pressures of O(2) or at low CO(2) concentrations. It is concluded that O(2) depresses photosynthesis primarily by causing a shift of a major portion of the total carbon into glycolate and impairing the functioning of the photosynthetic carbon reduction cycle. The mechanism whereby O(2) alters the flow of carbon into glycolate remains unknown.
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PMID:Inhibition of photosynthesis by oxygen in isolated spinach chloroplasts. 1665 76