Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:4.1.2.13 (
aldolase
)
3,461
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Glycine cleavage system (GCS) occupies a key position in one-carbon (C1) metabolic pathway and receives great attention for the use of C1 carbons like formate and CO
2
via synthetic biology. In this work, we demonstrate that formaldehyde exists as a substantial byproduct of the GCS reaction cycle. Three causes are identified for its formation. First, the principal one is the decomposition of
N
5
,N
10
-methylene-tetrahydrofolate (5,10-CH
2
-
THF
) to form formaldehyde and
THF
. Increasing the rate of glycine cleavage promotes the formation of 5,10-CH
2
-
THF
, thereby increasing the formaldehyde release rate. Next, formaldehyde can be produced in the GCS even in the absence of
THF
. The reason is that T-protein of the GCS can degrade methylamine-loaded H-protein (H
int
) to formaldehyde and ammonia, accompanied with the formation of dihydrolipoyl H-protein (H
red
), but the reaction rate is less than 0.16% of that in the presence of
THF
. Increasing T-protein concentration can speed up the release rate of formaldehyde by H
int
. Finally, a certain amount of formaldehyde can be formed in the GCS due to oxidative degradation of
THF
. Based on a formaldehyde-dependent
aldolase
, we elaborated a glycine-based one carbon metabolic pathway for the biosynthesis of 1,3-propanediol (1,3-PDO) in vitro. This work provides quantitative data and mechanistic understanding of formaldehyde formation in the GCS and a new biosynthetic pathway of 1,3-PDO.
...
PMID:Formaldehyde formation in the glycine cleavage system and its use for an aldolase-based biosynthesis of 1,3-prodanediol. 3246 27
Glycine cleavage system (GCS) occupies a key position in one-carbon (C1) metabolic pathway and receives great attention for the use of C1 carbons like formate and CO
2
via synthetic biology. In this work, we demonstrate that formaldehyde exists as a substantial byproduct of the GCS reaction cycle. Three causes are identified for its formation. First, the principal one is the decomposition of N
5
,N
10
-methylene-tetrahydrofolate (5,10-CH
2
-
THF
) to form formaldehyde and
THF
. Increasing the rate of glycine cleavage promotes the formation of 5,10-CH
2
-
THF
, thereby increasing the formaldehyde release rate. Next, formaldehyde can be produced in the GCS even in the absence of
THF
. The reason is that T-protein of the GCS can degrade methylamine-loaded H-protein (H
int
) to formaldehyde and ammonia, accompanied with the formation of dihydrolipoyl H-protein (H
red
), but the reaction rate is less than 0.16% of that in the presence of
THF
. Increasing T-protein concentration can speed up the release rate of formaldehyde by H
int
. Finally, a certain amount of formaldehyde can be formed in the GCS due to oxidative degradation of
THF
. Based on a formaldehyde-dependent
aldolase
, we elaborated a glycine-based one carbon metabolic pathway for the biosynthesis of 1,3-propanediol (1,3-PDO) in vitro. This work provides quantitative data and mechanistic understanding of formaldehyde formation in the GCS and a new biosynthetic pathway of 1,3-PDO.
...
PMID:Formaldehyde formation in the glycine cleavage system and its use for an aldolase-based biosynthesis of 1,3-propanediol. 3329 16
