Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:4.1.2.13 (
aldolase
)
3,461
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Ribose methylation is among the most ubiquitous modifications found in RNA. 2'-
O
-methyluridine is found in rRNA, snRNA, snoRNA and tRNA of
Archaea
,
Bacteria
, and
Eukaryota
. Moreover, 2'-
O
-methylribonucleosides are promising starting materials for the production of nucleic acid-based drugs. Despite the countless possibilities of practical use for the metabolic enzymes associated with methylated nucleosides, there are very few reports regarding the metabolic fate and enzymes involved in the metabolism of 2'-
O
-alkyl nucleosides. The presented work focuses on the cellular degradation of 2'-
O
-methyluridine. A novel enzyme was found using a screening strategy that employs
Escherichia coli
uracil auxotroph and the metagenomic libraries. A 2'-
O
-methyluridine hydrolase (RK9NH) has been identified together with an
aldolase
(RK9DPA)-forming a part of a probable gene cluster that is involved in the degradation of 2'-
O
-methylated nucleosides. The RK9NH is functional in
E. coli
uracil auxotroph and in vitro. The RK9NH
nucleoside hydrolase
could be engineered to enzymatically produce 2'-
O
-methylated nucleosides that are of great demand as raw materials for production of nucleic acid-based drugs. Moreover, RK9NH
nucleoside hydrolase
converts 5-fluorouridine, 5-fluoro-2'-deoxyuridine and 5-fluoro-2'-
O
-methyluridine into 5-fluorouracil, which suggests it could be employed in cancer therapy.
...
PMID:Identification of a 2'-
O
-Methyluridine Nucleoside Hydrolase Using the Metagenomic Libraries. 3040 65
