Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:4.1.2.13 (
aldolase
)
3,461
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Hypermucoviscosity phenotypic
Klebsiella pneumoniae
(HV-
Kp
) serotype K1 is the predominant pathogen of a pyogenic liver abscess, an
emerging infectious disease
that often complicates septic metastatic syndrome in diabetic patients with poor sugar control. HV-
Kp
isolates were more resistant to neutrophil phagocytosis than non-HV-
Kp
isolates because of different pathogen-associated molecular patterns. The protein expression of HV-
Kp
after interaction with neutrophils is unclear. We studied KP-M1 (HV phenotype; serotype K
1
), DT-X (an acapsularmutant strain of KP-M1), and
E. coli
(ATCC 25922) with the model of
Kp-
infected neutrophils, using a comparative proteomic approach. One the identified protein, namely fructose-1, 6-bisphosphate
aldolase
(FBA), was found to be distributed in the KP-M1 after infecting neutrophils. Cell fractionation experiments showed that FBA is localized both to the cytoplasm and the outer membrane. Flow cytometry demonstrated that outer membrane-localized FBA was surface-accessible to FBA-specific antibody. The
fba
gene expression was enhanced in high glucose concentrations, which leads to increasing bacterial resistance to neutrophils phagocytosis and killing. The KP-M1 after FBA inhibitors and FBA-specific antibody treatment showed a significant reduction in bacterial resistance to neutrophils phagocytosis and killing, respectively, compared to KP-M1 without treatment. FBA is a highly conserved surface-exposed protein that is required for optimal interaction of HV-
Kp
to neutrophils.
...
PMID:The Surface Protein Fructose-1, 6 Bisphosphate Aldolase of
Klebsiella pneumoniae
Serotype K1: Role of Interaction with Neutrophils. 3326 5
