Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:4.1.2.13 (
aldolase
)
3,461
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Using an improved procedure,
Z-protein
was prepared from myofibrils of chicken breast muscle. The yield of pure
Z-protein
increased to 10 mg per kg of muscle. The chain weight of
Z-protein
was 55,000 in the presence of SDS. However,
Z-protein
was eluted before
aldolase
(Mr 158,000) in Sephacryl S-400 column chromatography, and, therefore, it appeared to exist as a tetramer in a physiological salt solution.
Z-protein
had at least four isopeptides whose isoelectric points ranged from pH 6.0 to 6.4. Anti-
Z-protein
antiserum reacted equally with these isopeptides. The extinction coefficient of
Z-protein
at wavelength 278 nm was 4.2 (1%; light path, 1 cm).
Z-protein
which was purified according to this improved method did not bind to F-actin and alpha-actinin in a physiological salt solution.
...
PMID:Refined purification and characterization of Z-protein. 277 42
