Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:4.1.1.6 (
CAD
)
4,420
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The aim of this study was to investigate the changes in expression pattern of the most important genes connected with apoptosis in proliferative apoptotic lesions (hyperplasia, adenoma), applying cDNA microarray technique, in order to promote the possible diagnostic or therapeutic utilisation of any difference in gene expression compared to the healthy (normal) parathyroid gland. Samples were taken from surgically removed 2 hyperplasias, 2 adenomas and 2 normal parathyroid glands. The Apoptosis Gene Array (Superarray) was used. This contains 112 genes, in tetraspot arrangement. The probes measured 250-600 base pairs. Streptavidin was bound to the array. CDP Star TM chemiluminescent substrate was used for detection. The samples deriving from hyperplasia or adenoma were compared to samples from normal parathyroid glands. The following genes were overexpressed in both hyperplasia and adenoma: CHEK1, ATM, BCL-XL, FAS, TNF, cIAP1, TRAIL, FADD, CASP 4,5,6,8, CD120b, CD137, LTA, TANK, TARF2,
CAD
, LIGHTR, DR3LG. CASP1,10, BFAR, BOD, BCL2L2, TRANCE were underexpressed in both hyperplasia and adenoma. Genes overexpressed only in hyperplasia were: MDM2, MCL1, BCL2A1, BLK, RIPK2, CD40LG, TRAF5, HUS1, BNIP3. Underexpressed only in hyperplasia: BOK, CIDEA, TRAF1, TRIP. Overexpressed only in adenoma: APOLLON, RIPK1,
LTB
, LTBR, CASP2,13, cIAP2, CIDEB. Underexpressed only in adenoma: TRAF4 and FASLG. Overexpresion or underexpression meant 1.5-fold difference from normal average values. As a result of this study, both pro-apoptotic and antiapoptotic genes were identified in hyperplasia and adenoma of the parathyroid gland. It seems that increased proliferation is connected also with increased apoptotic activity, but tumor cell candidates are able to survive, by activation of signal pathways resulting in overexpresion of anti-apoptotic genes.
...
PMID:[Changes in gene expression in the course of proliferative processes in the parathyroid gland]. 1688 77
High-density lipoprotein (HDL) interacts with various cells, particularly macrophages, in functional cell-HDL interactions. Here, we found that HDL protein quality and lipid quality play critical roles in HDL functions. HDL fractions from healthy volunteers (HDL
Healthy
) and patients with recurrent coronary atherosclerotic disease (HDL
CAD
) were prepared. To analyse functional HDL-macrophage interactions, macrophages were co-incubated with each HDL, and lipid mediator production was assessed by liquid chromatography/mass spectrometry-based metabololipidomics. HDL
Healthy
treatment attenuated the pro-inflammatory lipid mediator production, particularly that of leukotriene (LT) B
4
, and this treatment enhanced lipoxin (LX) B
4
and resolvin (Rv) E2 production. HDL
Healthy
treatment enhanced the proteasome-mediated degradation of the
LTB
4
-producing enzyme 5-lipoxygenase (LO) in activated macrophages; however, HDL
CAD
did not show these anti-inflammatory effects. HDL
Healthy
was engulfed by macrophages via clathrin-mediated endocytosis, which was a critical step in 5-LO/
LTB
4
regulation. We also found that HDL
CAD
showed higher levels of the
LTB
4
-producing enzymes and thus promoted
LTB
4
production from HDL
CAD
. In addition,
LTB
4
attenuated HDL endocytosis, HDL-mediated 5-LO degradation in macrophages, and HDL-derived augmentation of macrophage phagocytosis. These results indicated that local
LTB
4
produced de novo from HDL
CAD
regulates HDL-macrophage functional interactions and plays critical roles in dysfunctional, inflammatory HDL characteristics.
...
PMID:Novel mechanism of regulation of the 5-lipoxygenase/leukotriene B
4
pathway by high-density lipoprotein in macrophages. 2902 82
