Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
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Target Concepts:
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Query: EC:4.1.1.49 (
phosphoenolpyruvate carboxykinase
)
4,654
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The hysteretic nature of
phosphoenolpyruvate carboxylase
from maize was investigated at pH 7 by (a) transient kinetic studies, (b) kinetics of inhibition by
2-PG
, a structural analog of PEP, and (c) effect of
2-PG
on equilibrium binding of Mg2+. The lag time as a function of substrate concentration was nonlinear with an oblique asymptote. During steady state, cooperative kinetics for Mg2+ was changed to hyperbolic kinetics in the presence of
2-PG
. Studies on the equilibrium binding of Mg2+ with the help of an external fluorescent probe, 8-anilino-6-naphthalinosulfonate showed that the hyperbolic binding of Mg2+ was changed to cooperative binding in the presence of
2-PG
. On the basis of these results along with the results presented in the preceding paper, a fully concerted sequential model with subunit interaction is proposed for PEPC.
...
PMID:Hysteretic nature of phosphoenolpyruvate carboxylase isolated from maize. 230 26
