Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:4.1.1.32 (
phosphoenolpyruvate carboxykinase
)
4,204
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The Saccharomyces cerevisiae nuclear gene
MRP-S9
was identified as part of the European effort in sequencing chromosome II.
MRP-S9
encodes for a hydrophilic and basic protein of 278 amino acids with a molecular mass of 32 kDa. The C-terminal part (aa 153-278) of the
MRP-S9
protein exhibits significant sequence similarity to members of the eubacterial and chloroplast S9 ribosomal-protein family. Cells disrupted in the chromosomal copy of
MRP-S9
were unable to respire and displayed a characteristic phenotype of mutants with defects in mitochondrial protein synthesis as indicated by a loss of cytochrome c oxidase activity. Additionally, no activities of the gluconeogenetic enzymes, fructose-1,6-bisphosphatase and
phosphoenolpyruvate carboxykinase
, could be observed under conditions of glucose de-repression. The respiration-deficient phenotype could not be restored by transformation of the disruption strain with a wild-type copy of
MRP-S9
, indicating that
MRP-S9
disruption led to rho- or rho0 cells. Sequence similarities of
MRP-S9
to other members of the ribosomal S9-protein family and the phenotype of disrupted cells are consistent with an essential role of
MRP-S9
is assembly and/or function of the 30s subunit of yeast mitochondrial ribosomes.
...
PMID:Cloning and analysis of the nuclear gene MRP-S9 encoding mitochondrial ribosomal protein S9 of Saccharomyces cerevisiae. 853 10
