Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:4.1.1.32 (phosphoenolpyruvate carboxykinase)
 4,204 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Pyruvate orthophosphate dikinase (PPDK) is known for its role in C4 photosynthesis but has no established function in C3 plants. Abscisic acid, PEG and submergence were found to markedly induce a protein of about 97 kDa, identified by microsequencing as PPDK, in rice roots (C3). The rice genome was found to contain two ppdk loci, osppdka and osppdkb. We isolated osppdka cDNA, which encodes a cytosolic rice PPDK isoform of 96.6 kDa, that corresponded to the ABA-induced protein from roots. Western blot analysis showed a PPDK induction in roots of rice seedlings during gradual drying, cold, high salt and mannitol treatment, indicating a water deficit response. PPDK was also induced in the roots and sheath of submerged rice seedlings, and in etiolated rice seedlings exposed to an oxygen-free N2 atmosphere, which indicated a low-oxygen stress response. None of the stress treatments induced PPDK protein accumulation in the lamina of green rice seedlings. Ppdk transcripts were found to accumulate in roots of submerged seedlings, concomitant with the induction of alcohol dehydrogenase 1. Low-oxygen stress triggered an increase in PPDK activity in roots and etiolated rice seedlings, accompanied by increases in phosphoenolpyruvate carboxylase and malate dehydrogenase activities. The results indicate that cytosolic PPDK is involved in a metabolic response to water deficit and low-oxygen stress in rice, an anoxia-tolerant species.
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PMID:Low-oxygen stress and water deficit induce cytosolic pyruvate orthophosphate dikinase (PPDK) expression in roots of rice, a C3 plant. 974 98

The regulatory Lc gene is a member of the R gene family of maize which amongst other transcriptional activators controls anthocyanin biosynthetic genes. The availability of R locus mutants which lack anthocyanin production in all tissues offers the possibility of studying cell lineage by introducing a chimeric Lc gene into defined cells and following cell autonomous anthocyanin production. For this purpose integrative and self-replicating Lc vectors were constructed. Integrative expression vectors contained the 2.4 kbp Lc cDNA with the entire 5' leader fused to the constitutive cauliflower mosaic virus 35S promoter with and without the maize alcohol dehydrogenase 1 intron 1 or to the mesophyll specific phosphoenolpyruvate carboxylase promoter of maize. To enhance expression and to circumvent the necessity of stable integration, extrachromosomally replicating and expressing wheat dwarf virus-Lc constructions were also designed. Both categories of expression vectors were tested in embryogenic callus-derived protoplasts of maize and were found to transactivate anthocyanin biosynthesis. Southern blot analysis indicated that the wheat dwarf virus-Lc constructions were replicating.
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PMID:Integrative and self-replicating Lc vectors and their transactivation capacity in maize callus protoplasts. 2419 22