Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:4.1.1.17 (
ornithine decarboxylase
)
6,351
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
A rapid and sensitive spectrophotometric assay for
ornithine decarboxylase
is described. It is based on the observation that the product of
ornithine decarboxylase
, putrescine, reacts with 2,4,6-trinitrobenzenesulfonic acid to give a colored product soluble in
1-pentanol
whereas ornithine does not. The amount of putrescine produced by the enzyme was determined by measuring the absorbance of the
1-pentanol
extract of the reaction mixture at 420 nm, and by comparing the results to those obtained by the trapping of 14CO2 and by HPLC assays. The three assays were found to be equivalent in sensitivity, with the spectrophotometric assay having the advantages of being relatively rapid, requiring only common laboratory equipment, and not requiring the use of radioactive isotopes.
...
PMID:Spectrophotometric assay for ornithine decarboxylase. 357 55
