Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:4.1.1.17 (ornithine decarboxylase)
 6,351 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

In mouse FM3A ornithine decarboxylase (ODC) overproducing cells (EXOD-1), the amount of ODC protein was approximately 100-fold that of normal cells. Since it is well known that the translational efficiency of ODC mRNA is very low and that eIF-4E is a limiting factor for the mRNA recognition and the scanning of 40 S ribosomal subunits, we measured the amount and phosphorylation of eIF-4E in EXOD-1 cells. An increase in the phosphorylation of eIF-4E, its association with p220 protein, and an enhancement of RNA helicase activity were observed in the cells. These results support the hypothesis that phosphorylation of eIF-4E enhances RNA helicase activity through eIF-4F (4A, 4E, and p220) complex formation.
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PMID:Enhancement of helicase activity and increase of eIF-4E phosphorylation in ornithine decarboxylase-overproducing cells. 865 16

Translation initiation plays an important role in cell growth, proliferation, and survival. The translation initiation factor eIF4B (eukaryotic initiation factor 4B) stimulates the RNA helicase activity of eIF4A in unwinding secondary structures in the 5' untranslated region (5'UTR) of the mRNA in vitro. Here, we studied the effects of eIF4B depletion in cells using RNA interference (RNAi). In agreement with the role of eIF4B in translation initiation, its depletion resulted in inhibition of this step. Selective reduction of translation was observed for mRNAs harboring strong to moderate secondary structures in their 5'UTRs. These mRNAs encode proteins, which function in cell proliferation (Cdc25C, c-myc, and ODC [ornithine decarboxylase]) and survival (Bcl-2 and XIAP [X-linked inhibitor of apoptosis]). Furthermore, eIF4B silencing led to decreased proliferation rates, promoted caspase-dependent apoptosis, and further sensitized cells to camptothecin-induced cell death. These results demonstrate that eIF4B is required for cell proliferation and survival by regulating the translation of proliferative and prosurvival mRNAs.
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PMID:Control of cell survival and proliferation by mammalian eukaryotic initiation factor 4B. 2008