Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:4.1.1.17 (
ornithine decarboxylase
)
6,351
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The mechanism of synthesis of
ornithine decarboxylase
(
ODC
) at the level of translation was studied using cell culture and cell-free systems. Synthesis of firefly luciferase (Fluc) from the second open reading frame (ORF) in a bicistronic construct transfected into FM3A and HeLa cells was enhanced by the presence of the 5'-untranslated region (5'-UTR) of
ODC
mRNA between the two ORFs. However, cotransfection of the gene encoding
2A protease
inhibited the synthesis of Fluc. Synthesis of Fluc from the second cistron in the bicistronic mRNA in a cell-free system was not affected significantly by the 5'-UTR of
ODC
mRNA. Synthesis of
ODC
from
ODC
mRNA in a cell-free system was inhibited by
2A protease
and cap analogue (m7GpppG). Rapamycin inhibited
ODC
synthesis by 40-50% at both the G1/S boundary and the G2/M phase. These results indicate that an IRES in the 5'-UTR of
ODC
mRNA does not function effectively.
...
PMID:Minor contribution of an internal ribosome entry site in the 5'-UTR of ornithine decarboxylase mRNA on its translation. 1792 56
